IL-20R alpha Antibody (MM0394-9F40) - Azide and BSA Free

Immunohistochemistry-Paraffin: IL-20 R alpha Antibody (MM0394-9F40) [NBP2-11695] - 10% Buffer formalin fixed and parffine embedded human placetal tissue section (4um) is subjected IHC staining using NBP2-11695. Tissue section was pretreated in citric buffer (pH 6.0) with microwave for antige retrieval before IHC is applied.

PDAC cell lines. (A) Laser scan microscopy and Western blotting of the PDAC cell lines AsPC-1, BxPC-3, and Panc-1 incubated with anti-IL10RB and anti-IL20RA antibodies shows expression and colocalization of both receptors in all three cell lines. (B) Western blotting of PDAC cells shows IL26 induced phosphorylation of STAT3 (left panel) and ERK1/2 (right panel) in a time-dependent manner. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/31948053), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clone: MM0394-9F40
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

IL-20R alpha Antibody (MM0394-9F40) - Azide and BSA Free Summary

• Immunogen: Human recombinant IL-20R alpha (extracellular domain)
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: IL20RA
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:20-100
  • Immunohistochemistry-Paraffin 1:20-1:100
  • Western Blot 1:500-1:1000
• Application Notes: Use in Immunocytochemistry/Immunofluorescence reported in scientific literature (PMID:31948053).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS. 0.025 mg size is provided in liquid form, PBS
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein G purified
• Reconstitution Instructions: Reconstitute with sterilized PBS to a final concentration of 0.5 mg/ml.

Alternate Names for IL-20R alpha Antibody (MM0394-9F40) - Azide and BSA Free

• class II cytokine receptor ZCYTOR7
• CRF2-8
• Cytokine receptor class-II member 8
• Cytokine receptor family 2 member 8
• FLJ40993
• IL-20 R alpha
• IL20R alpha
• IL-20R alpha
• IL-20R1
• IL-20R1IL-20 receptor subunit alpha
• IL20RA
• IL-20Ra
• IL-20R-alpha
• interleukin 20 receptor, alpha
• interleukin-20 receptor I
• interleukin-20 receptor subunit alpha
• zcytor7

Background

Official Gene Symbol: IL20RA Gene ID: 53832 (Human) Gene Map Locus: 6q22.33-q23.1 (Human) IL20RA, is a subunit of IL20R, a member of Cytokine Class II receptor super family. It consists of a central transmembrane domain and functionally induces IL20 and IL26 signaling in epithelial cells and keratinocytes. It associates with IL-20RB and forms a functional receptor complex for IL20, IL19, and IL24. It also associates with IL-10RB and forms a unique heterodimeric functional receptor complex for IL-26. Upon ligand binding, it induces signaling through activation of STAT3 and STAT1. IL20RA is specifically expressed in skin suggesting a prominent role in growth and proliferation of keratinocytes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-20R alpha Antibody (NBP2-11695)(1)

Publication using NBP2-11695

• Mayer P, Linnebacher A, Glennemeier-Marke H et al. The Microarchitecture of Pancreatic Cancer as Measured by Diffusion-Weighted Magnetic Resonance Imaging Is Altered by T Cells with a Tumor Promoting Th17 Phenotype Int J Mol Sci 2020-01-05 [PMID: 31948053] (WB, IF/IHC, IHC-P, ICC/IF, Human)

Bioinformatics

• Gene Symbol: IL20RA
• Uniprot:
  • Q9UHF4()