Human IL-20 ELISA Kit (Colorimetric)

Product Discontinued

Human IL-20 ELISA Kit (Colorimetric) Summary

• Description: Quality control test: Standard curve. IL20 (Human) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of human IL20.
• Standard Curve Range: 16 to 4000 pg/mL
• Sensitivity: n/a
• Assay Type: Sandwich ELISA
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: IL20

Applications/Dilutions

• Dilutions:
  • ELISA
  • Quantification
• Application Notes: Useful for quantification.

Packaging, Storage & Formulations

• Storage: Store at 4C.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Human IL-20 ELISA Kit (Colorimetric)

• Cytokine Zcyto10
• IL10D
• IL20
• IL-20
• IL-20ZCYTO10four alpha helix cytokine
• interleukin 20
• interleukin-20
• MGC96907

Background

The class II cytokine receptor family includes 12 members, 11 of which combine as various heterodimers to transduce signals across the cellular membrane for 27 cytokines. These cytokines are divided into four structurally related groups: six cytokines of the IL-10 family, 17 type I IFNs, one type II IFN and three IFN-lamda. IL-20 is a member of the IL-10 family of cytokines that has been shown to be upregulated in psoriatic skin. The receptor for IL-20 (IL-20R) exists as a dimer consisting of IL-20Ralpha and IL-20Rbeta. IL-20R also functions as a receptor for IL-19, IL-24 and IL-26. It is highly expressed in skin, brain and testis. IL-20, IL-20Ralpha and IL-20Rbeta all have higher expression levels in psoriatic lesional (LS) skin than in nonlesional (NL) skin, suggesting a role for these proteins as potential therapeutic targets. IL-20 can induce signal transducer and activator of transcription (Stat) activation through IL-20R.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for IL-20 ELISA Kit (KA2191). (Showing 1 - 2 of 2 FAQ).

1. What is the time of incubation for coloration?
   • This is a product we distribute for Abnova. According to their protocol, they do not recommend a specific time for incubation, but suggest monitoring your wells for color change. See excerpt below: After the 4th wash step, add 100 ul of TMB substrate solution into each well and incubate at room temperature for color development. The microplate should be kept out of direct light by either covering with an opaque object or putting it into a dark room. Closely monitor the color development as some wells may turn blue very quickly depending on analyte and/or detection antibody-HRP concentrations. Once the blue color has ceased to develop further, immediately add 100 ul of Stop Solution to each well being used. The color in the wells should immediately change from blue to yellow. In general these color developments typically take about 5-10 minutes. Here is a link to Abnova's full protocol for your reference.
2. wondering what the difference is between your quantikine and duo set elisas?
   • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

Bioinformatics

• Gene Symbol: IL20