IL-19 Antibody (350105) [Alexa Fluor® 700]

• Reactivity: Mu
• Applications: Flow
• Clone: 350105
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Alexa Fluor 700
• Concentration: Please see the vial label for concentration. If unlisted please contact technical services.

IL-19 Antibody (350105) [Alexa Fluor® 700] Summary

• Immunogen: E. coli-derived recombinant mouse IL-19
  Leu25-Ala176
  Accession # Q8CJ70
• Specificity: Detects mouse IL-19 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human
  IL-19 is observed.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Gene: IL19
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Intracellular Staining by Flow Cytometry 0.25-1 ug/10^6 cells
• Application Notes: Flow Cytometry: Please use 0.25-1 ug of conjugated antibody per 10e6 cells.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
• Preservative: 0.09% Sodium Azide
• Concentration: Please see the vial label for concentration. If unlisted please contact technical services.

Notes

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-19 Antibody (350105) [Alexa Fluor® 700]

• IL-10C
• IL19
• IL-19
• interleukin 19
• MDA1
• melanoma differentiation associated protein-like protein
• NG.1Melanoma differentiation-associated protein-like protein
• ZMDA1interleukin-19

Background

Interleukin 19 (IL-19) is a member of the IL-10 family of cytokines (1). The IL-10 family is a class II alpha -helical collection of cytokines that contains two groups, a viral homolog and a cellular homolog group. Within the cellular homolog group, there are two additional groupings, one which uses IL-10 R2 as a signal transducing receptor (IL-10, IL-22 and IL-26), and one which uses IL-20 R2 as a signal transducing receptor (IL-19, IL-20 and IL-24) (2‑4). Mouse IL-19 is synthesized as a 176 amino acid (aa) precursor that contains a 24 aa signal sequence and a 152 aa mature region (5). Based on human studies, it is expected to be secreted as a glycosylated monomer, 35‑45 kDa in size (2, 6, 7). IL-19 is unusual in that it contains seven amphipathic helices (2, 4, 8). Mature mouse IL-19 shares 69% aa sequence identity with the mature human IL-19, and 85% and 68% aa identity to unpublished Genbank sequences for rat and canine IL-19, respectively. Although mouse IL-19 is active on human cells, human IL-19 is not active on mouse cells (5). IL-19 expression is limited to activated keratinocytes and monocytes, with a possible contribution from B cells (6, 9, 10). IL-19 binds a receptor complex consisting of the IL-20 receptor alpha (also known as IL-20 R1) and the IL-20 receptor beta (IL-20 R2) (3, 4, 11, 12). This receptor complex is also shared by IL-20 and IL-24. Notably, IL-19 is reported to actually bind to IL-20 R2, which is generally considered to be only the signal transducing receptor subunit (7, 13). Functionally, it has been reported that IL-19 both will and will not induce IL-6 and TNF production by monocytes (5, 14). It does, however, seem to drive T-helper cell differentiation towards a Th2 response, inducing both IL-10 and production of itself (5, 14, 15).

1. Fickenscher, H. et al. (2002) Trends Immunol. 23:89.  
2. Pestka, S. et al. (2004) Annu. Rev. Immunol. 22:929. 
3. Zdanov (2004) Curr. Pharm. Des. 10:3873. 
4. Gallagher, G. et al. (2004) Int. Immunopathol. 4:615.  
5. Laio, Y.-C. et al. (2002) J. Immunol. 169:4288. 
6. Gallagher, G. et al. (2000) Genes Immun. 1:442. 
7. Pletnev, S. et al. (2003) Biochemistry 42:12617. 
8. Chang, C. et al. (2003) J. Biol. Chem. 278:3308.
9. Romer, J. et al. (2003) J. Invest. Dermatol. 121:1306.
10. Wolk, K. et al. (2002) J. Immunol. 168:5397.
11. Dumoutier, L. et al. (2001) J. Immunol. 167:3545.
12. Parrish-Novak, J. et al. (2002) J. Biol. Chem. 277:47517
13. Preimel, D. and H. Sticht (2004) J. Mol. Model. 10:290.
14. Jordan, W.J. et al. (2005) Eur. J. Immunol. 35:1576.
15. Laio, S.-C. et al. (2004) J. Immunol. 173:6712.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IL19
• Uniprot:
  • Q8CJ70()