IL-10 R beta Antibody (547324) [Unconjugated]

EL‑4 mouse lymphoblast cell line was stained with Rat Anti-Mouse IL‑10 R beta Monoclonal Antibody (Catalog # MAB53681, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG F(ab')2 Secondary Antibody (Catalog # F0113).

• Reactivity: Mu
• Applications: Flow, CyTOF-ready
• Clone: 547324
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

IL-10 R beta Antibody (547324) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse IL-10 R beta
  Ile21-Ser220
  Accession # Q61190
• Specificity: Detects mouse IL-10 R beta in direct ELISAs.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: IL10RB
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-10 R beta Antibody (547324) [Unconjugated]

• CDw210b antigen
• CDw210b
• CRF2-4IL-10R subunit 2
• CRFB4
• CRFB4human transmembrane receptor protein; cytokine receptor10CDW210B
• D21S58
• D21S66
• IBD25
• IL-10 R beta
• IL-10 receptor subunit beta
• IL10R beta
• IL-10R2
• IL-10R2IL-10R subunit beta
• IL10RB
• IL-10Rb
• interleukin 10 receptor, beta
• Interleukin-10 receptor subunit 2
• interleukin-10 receptor subunit beta
• member 4

Background

IL-10 R beta (Interleukin 10 receptor beta; also IL-10R2, CD210b and CRF2-4) is an 80-85 kDa member of the type II cytokine receptor family of proteins. It is very widely expressed and serves as a signal transducing accessory chain when complexed to the ligand-binding chains for IL-10, -22, -28A, -28B, and -29 (plus IL-26 in human). Mature mouse IL-10 R beta is a type I transmembrane protein with an extracellular domain that possesses two fibronectin type III domains (aa 22-107 and
111‑208). There is one alternate start site at Met20. Over aa 21- 220, mouse IL-10 R beta shares 87% and 74% aa identity with rat and human IL-10 R beta , respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-10 R beta Antibody (MAB53681)(1)

Publication using MAB53681

• S Mossner, M Kuchner, N Fazel Moda, B Knebel, H Al-Hasani, DM Floss, J Scheller Synthetic interleukin 22 (IL-22) signaling reveals biological activity of homodimeric IL-10R2 and functional cross-talk with the IL-6 receptor gp130 J. Biol. Chem., 2020-07-01;0(0):. 2020-07-01 [PMID: 32611765] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: IL10RB
• Uniprot:
  • Q61190()