IGSF4C/SynCAM4 Antibody

Western Blot: IGSF4C/SynCAM4 Antibody [NBP1-00164] - Analysis of Cell adhesion molecule 4 using NBP1-00164 in Human Brain (Cerebellum) lysate (35 ug protein in RIPA buffer) at 0.1 ug/ml.

Immunohistochemistry-Paraffin: IGSF4C/SynCAM4 Antibody [NBP1-00164] - Staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Immunohistochemistry-Paraffin: IGSF4C/SynCAM4 Antibody [NBP1-00164] - (2.5ug/ml) staining of paraffin embedded Human Thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

IGSF4C/SynCAM4 Antibody Summary

• Immunogen: Peptide with sequence C-KDERFQLEEFS corresponding to internal region according to NP_660339.1.
• Predicted Species: Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CADM4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 2 - 5 ug/ml
  • Immunohistochemistry-Paraffin 1:10 - 1:500
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 0.1 - 0.3 ug/ml
• Application Notes: WB: Approx. 70 kDa band observed in human brain (cerebellum) lysates (calculated MW of 42.8 kDa band according to NP_660339.1). The observed molecular weight corresponds to findings with NB100-93383, a product of different design reacting to a different part of the same protein. IHC-P: Human thymus and skeletal muscle shows staining of adhesion sites between medullar cells.
• Control:

  IGSF4C/SynCAM4 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for IGSF4C/SynCAM4 Antibody

• CADM4
• cell adhesion molecule 4
• IGSF4C
• IGSF4Cnectin-like 4
• Immunoglobulin superfamily member 4C
• immunoglobulin superfamily, member 4C
• Necl-4
• NECL4synCAM4
• Nectin-like protein 4
• SynCAM4
• TSLC1-like 2
• TSLC1-like protein 2
• TSLL2
• TSLL2SynCAM4

Background

Cell adhesion molecule 4 is involved in the cell-cell adhesion. Has calcium- and magnesium-independent cell-cell adhesion activity. Mayhave tumor-suppressor activity

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IGSF4C/SynCAM4 Antibody (NBP1-00164)(1)

Publication using NBP1-00164

• Spiegel I, Adamsky K, Eshed Y et al. A central role for Necl4 (SynCAM4) in Schwann cell-axon interaction and myelination. Nat Neurosci 2007-07-01 [PMID: 17558405]

Bioinformatics

• Gene Symbol: CADM4
• Entrez:
  • 199731(Human)
  • 260299(Mouse)
  • 365216(Rat)
• Uniprot:
  • NP_660339.1(Human)