IGSF4C/SynCAM4 Antibody

Western Blot: IGSF4C/SynCAM4 Antibody [NB100-93383] - Staining of Human Brain (Cerebellum) 35ug protein in RIPA buffer). A) (0.1ug/ml) and B) (0.05ug/ml). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: IGSF4C/SynCAM4 Antibody [NB100-93383] - (2.5ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Immunohistochemistry-Paraffin: IGSF4C/SynCAM4 Antibody [NB100-93383] - (2.5ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Mu, Rt, Ca
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

IGSF4C/SynCAM4 Antibody Summary

• Immunogen: Peptide with sequence C-REAFLNGSDGHKRKE corresponding to C-Terminus according to NP_660339.1.
• Epitope: C-REAFLNGSDGHKRKE
• Predicted Species: Mouse (100%), Rat (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CADM4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 1:100-1:2000
• Application Notes: WB: Approx. 70 kDa band observed in human brain (cerebellum) lysates (calculated MW of 42.8 kDa band according to NP_660339.1). The observed molecular weight corresponds to findings with NBP1-00164, a product of different design reacting to a different part of the same protein.
• Control:

  IGSF4C/SynCAM4 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for IGSF4C/SynCAM4 Antibody

• CADM4
• cell adhesion molecule 4
• IGSF4C
• IGSF4Cnectin-like 4
• Immunoglobulin superfamily member 4C
• immunoglobulin superfamily, member 4C
• Necl-4
• NECL4synCAM4
• Nectin-like protein 4
• SynCAM4
• TSLC1-like 2
• TSLC1-like protein 2
• TSLL2
• TSLL2SynCAM4

Background

Cell adhesion molecule 4 is involved in the cell-cell adhesion. Has calcium- and magnesium-independent cell-cell adhesion activity. Mayhave tumor-suppressor activity

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IGSF4C/SynCAM4 Antibody (NB100-93383)(1)

Publication using NB100-93383

• Spiegel I, Adamsky K, Eshed Y et al. A central role for Necl4 (SynCAM4) in Schwann cell-axon interaction and myelination. Nat Neurosci 2007-07-01 [PMID: 17558405]

Bioinformatics

• Gene Symbol: CADM4
• Entrez:
  • 199731(Human)
  • 260299(Mouse)
  • 365216(Rat)
• Uniprot:
  • NP_660339.1(Human)