![SDS-Page: Rat IgM Isotype Control [HRP] [NBP1-97203] - Lane M: 3 uL Opal Prestained Marker. Lane 1: Reduced Rat IgG Whole Molecule. Lane 2: Reduced Rat IgG F(c) Fragment. Lane 3: Reduced Rat IgG Fab Fragment. Lane 4: Reduced Rat IgM Whole Molecule Peroxidase Conjugated. Load: 1 ug of IgG, F(c), Fab; 1.5 ug of IgM.](http://images.novusbio.com/fullsize2/Rat-IgM-Isotype-Control-[HRP]-SDS-Page-NBP1-97203-img0001.jpg "SDS-Page: Rat IgM Isotype Control [HRP] [NBP1-97203] - Lane M: 3 uL Opal Prestained Marker. Lane 1: Reduced Rat IgG Whole Molecule. Lane 2: Reduced Rat IgG F(c) Fragment. Lane 3: Reduced Rat IgG Fab Fragment. Lane 4: Reduced Rat IgM Whole Molecule Peroxidase Conjugated. Load: 1 ug of IgG, F(c), Fab; 1.5 ug of IgM.")
| Reactivity | RtSpecies Glossary |
| Applications | PAGE |
| Host | Rat |
| Conjugate | HRP |
| Format | Purified |
| Concentration | LYOPH |
| Description | This Rat IgM immunoglobulin is useful as an isotype control for Rat IgM antibody. |
| Specificity | Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti- Rat IgM and anti-Rat Serum. |
| Isotype | IgM |
| Host | Rat |
| Purity | Size Exclusion Chromatography |
| Storage | Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles. |
| Buffer | 0.04 M Potassium Phosphate, 0.30 M Sodium Chloride, pH 7.2, 10 mg/mL (BSA) - Immunoglobulin and Protease free |
| Preservative | 0.01% Gentamicin Sulfate |
| Concentration | LYOPH |
| Purity | Size Exclusion Chromatography |
| Reconstitution Instructions | Reconstitute with 0.5 ml deionized water. |
![Rat IgM Isotype Control [HRP]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "Rat IgM Isotype Control [HRP]")
Diseases for Rat IgM Isotype Control (NBP1-97203)Discover more about diseases related to Rat IgM Isotype Control (NBP1-97203).
| Pathways for Rat IgM Isotype Control (NBP1-97203)View related products by pathway.
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Understanding the Importance of Human IgG Human IgG is a component of the immune system that protects the body from infection. It is the most abundantly found antibody isotype within the circulatory system of the human body. All antibody isotypes contain two heavy chains and two light chains... Read full blog post. |
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![Western Blot: MLC1 Antibody [NBP1-80073] - Titration: 0.2-1 ug/ml, Positive Control: Human Spleen.](http://images.novusbio.com/fullsize2/MLC1-Antibody-Western-Blot-NBP1-80073-img0003.jpg)
![Immunohistochemistry-Paraffin: MLC1 Antibody [NBP1-80073] - Rat Astrocytes, 20 ug/ml.](http://images.novusbio.com/fullsize2/MLC1-Antibody-Immunohistochemistry-Paraffin-NBP1-80073-img0002.jpg)
![Western Blot: MLC1 Antibody [NBP1-80073] - Human Fetal Spleen, Antibody Dilution: 1.0 ug/ml.](http://images.novusbio.com/fullsize2/MLC1 Antibody-Western Blot-NBP1-80073-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: RUNX1T1/ETO Antibody [NBP2-55747] - Staining of human cell line HEK 293 shows localization to nucleoplasm.](http://images.novusbio.com/fullsize2/RUNX1T1-ETO Antibody-Immunocytochemistry-Immunofluorescence-NBP2-55747-img0001.jpg)
![Western Blot: Aldo-keto Reductase 1C4/AKR1C4 Antibody (2C11) [H00001109-M01] - AKR1C4 monoclonal antibody (M01), clone 2C11 Analysis of AKR1C4 expression in MCF-7.](http://images.novusbio.com/fullsize2/Aldo-keto-Reductase-1C4-AKR1C4-Antibody-(2C11)-Western-Blot-H00001109-M01-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: Aldo-keto Reductase 1C4/AKR1C4 Antibody (2C11) [H00001109-M01] - Analysis of monoclonal antibody to AKR1C4 on HepG2 cell. Antibody concentration 15 ug/ml.](http://images.novusbio.com/fullsize2/Aldo-keto-Reductase-1C4-AKR1C4-Antibody-(2C11)-Immunocytochemistry-Immunofluorescence-H00001109-M01-img0011.jpg)
![Immunohistochemistry-Paraffin: Aldo-keto Reductase 1C4/AKR1C4 Antibody (2C11) [H00001109-M01] - Analysis of monoclonal antibody to AKR1C4 on formalin-fixed paraffin-embedded human liver tissue. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize2/Aldo-keto-Reductase-1C4-AKR1C4-Antibody-(2C11)-Immunohistochemistry-Paraffin-H00001109-M01-img0013.jpg)
![Western Blot: beta-Galactosidase-1/GLB1 Antibody (1C9) [NBP2-45731] - Analysis of extracts (35ug) from 9 different cell lines by using anti-GLB1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize2/beta-Galactosidase-1-GLB1-Antibody-(1C9)-Western-Blot-NBP2-45731-img0016.jpg)
![Immunohistochemistry: beta-Galactosidase-1/GLB1 Antibody (1C9) [NBP2-45731] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize2/beta-Galactosidase-1-GLB1-Antibody-(1C9)-Immunohistochemistry-NBP2-45731-img0013.jpg)
![Western Blot: beta-Galactosidase-1/GLB1 Antibody (1C9) [NBP2-45731] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GLB1.](http://images.novusbio.com/fullsize2/beta-Galactosidase-1-GLB1-Antibody-(1C9)-Western-Blot-NBP2-45731-img0014.jpg)
![Western Blot: CDR2 Antibody [NB110-58345] - Analysis of CDR2 antibody on HeLa whole cell.](http://images.novusbio.com/fullsize2/CDR2-Antibody-Western-Blot-NB110-58345-img0006.jpg)
![Immunohistochemistry: CDR2 Antibody [NB110-58345] - Purkinje neurons of the cerebellum.](http://images.novusbio.com/fullsize2/CDR2-Antibody-Immunohistochemistry-NB110-58345-img0004.jpg)
![Flow Cytometry: CDR2 Antibody [NB110-58345] - Analysis using the DyLight 488 conjugate of NB110-58345. Thymus epithelial cells CDR2. Image from verified customer review.](http://images.novusbio.com/fullsize2/CDR2-Antibody-Flow-Cytometry-NB110-58345-img0008.jpg)
![Western Blot: CD5 Antibody (7A7) [NBP2-02690] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD5.](http://images.novusbio.com/fullsize/CD5 Antibody (7A7)-Western Blot-NBP2-02690-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: CD5 Antibody (7A7) [NBP2-02690] Staining of COS7 cells transiently transfected by pCMV6-ENTRY CD5.](http://images.novusbio.com/fullsize/CD5 Antibody (7A7)-Immunocytochemistry-Immunofluorescence-NBP2-02690-img0008.jpg)
![Immunohistochemistry-Paraffin: CD5 Antibody (7A7) [NBP2-02690] - Staining of paraffin-embedded Human Kidney tissue using anti-CD5 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CD5 Antibody (7A7)-Immunohistochemistry-Paraffin-NBP2-02690-img0007.jpg)
![Western Blot: Glutathione S Transferase kappa 1 Antibody [NBP1-89727] - Lane 1: Marker [kDa] 229, 112, 84, 48, 32, 27, 17. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize2/Glutathione-S-Transferase-kappa-1-Antibody-Western-Blot-NBP1-89727-img0004.jpg)
![Immunohistochemistry-Paraffin: Glutathione S Transferase kappa 1 Antibody [NBP1-89727] - Staining in human small intestine and pancreas tissues using anti-GSTK1 antibody. Corresponding GSTK1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize2/Glutathione-S-Transferase-kappa-1-Antibody-Immunohistochemistry-Paraffin-NBP1-89727-img0007.jpg)
![Immunohistochemistry-Paraffin: Glutathione S Transferase kappa 1 Antibody [NBP1-89727] - Staining of human duodenum shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize2/Glutathione-S-Transferase-kappa-1-Antibody-Immunohistochemistry-Paraffin-NBP1-89727-img0002.jpg)
![Immunohistochemistry-Paraffin: SLCO6A1 Antibody [NBP2-49118] - Staining of human testis shows high expression.](http://images.novusbio.com/fullsize2/SLCO6A1-Antibody-Immunohistochemistry-Paraffin-NBP2-49118-img0004.jpg)
![Immunohistochemistry-Paraffin: SLCO6A1 Antibody [NBP2-49118] - Staining of human endometrium shows low expression as expected.](http://images.novusbio.com/fullsize2/SLCO6A1-Antibody-Immunohistochemistry-Paraffin-NBP2-49118-img0003.jpg)
![Immunohistochemistry-Paraffin: SLCO6A1 Antibody [NBP2-49118] - Staining in human testis and endometrium tissues using anti-SLCO6A1 antibody. Corresponding SLCO6A1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize2/SLCO6A1 Antibody-Immunohistochemistry-Paraffin-NBP2-49118-img0005.jpg)
![Western Blot: MKP-1/DUSP1 Antibody [NB100-834] - Probing MKP-1 with LV heart tissue from rats. This image was submitted via customer review.](http://images.novusbio.com/fullsize2/MKP-1-DUSP1-Antibody-Western-Blot-NB100-834-img0005.jpg)
![Immunohistochemistry-Paraffin: MKP-1/DUSP1 Antibody [NB100-834] - (3.8ug/ml) staining of Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize2/MKP-1-DUSP1-Antibody-Immunohistochemistry-Paraffin-NB100-834-img0003.jpg)
![Western Blot: MKP-1/DUSP1 Antibody [NB100-834] - (0.3ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize2/MKP-1-DUSP1-Antibody-Western-Blot-NB100-834-img0004.jpg)
![Western Blot: HLA DQA1 Antibody [NBP1-84550] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Human cell line Daudi.](http://images.novusbio.com/fullsize2/HLA-DQA1-Antibody-Western-Blot-NBP1-84550-img0004.jpg)
![Immunohistochemistry-Paraffin: HLA DQA1 Antibody [NBP1-84550] - Staining of human lung shows high expression.](http://images.novusbio.com/fullsize2/HLA-DQA1-Antibody-Immunohistochemistry-Paraffin-NBP1-84550-img0006.jpg)
![Immunohistochemistry-Paraffin: HLA DQA1 Antibody [NBP1-84550] - Staining of human tonsil shows strong cytoplasmic positivity in reaction center cells and lymphoid cells outside reaction centra.](http://images.novusbio.com/fullsize2/HLA-DQA1-Antibody-Immunohistochemistry-Paraffin-NBP1-84550-img0002.jpg)
![Western Blot: NOD2 Antibody (2D9) [NB100-524] - Total protein from human THP1 and US-OS cells and mouse Raw264.7 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-NOD2 in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize2/NOD2-Antibody-(2D9)-Western-Blot-NB100-524-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: NOD2 Antibody (2D9) [NB100-524] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-NOD2 (2D9) NB100-524 at a 1:200 dilution overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was counterstained with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize2/NOD2-Antibody-(2D9)-Immunocytochemistry-Immunofluorescence-NB100-524-img0008.jpg)
![Flow Cytometry: NOD2 Antibody (2D9) [NB100-524] - An intracellular stain was performed on Jurkat cells with NOD2 (2D9) antibody NB100-524APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes.](http://images.novusbio.com/fullsize2/NOD2-Antibody-(2D9)-Flow-Cytometry-NB100-524-img0014.jpg)
![Western Blot: Complement C4b/d Antibody (16D2) [NB200-541] - Western blot analysis of C4 expression in J774.A1 (A) aand mouse PBL (B) whole cell lysates.](http://images.novusbio.com/fullsize2/Complement-C4b-d-Antibody-(16D2)-Western-Blot-NB200-541-img0001.jpg)
![Western Blot: ARC/NOL3 Antibody [NBP2-19561] - Sample (30 ug of whole cell lysate) A: HeLa 12% SDS PAGE diluted at 1:1000](http://images.novusbio.com/fullsize2/ARC-NOL3-Antibody-Western-Blot-NBP2-19561-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: ARC/NOL3 Antibody [NBP2-19561] - Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using NOL3 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.](http://images.novusbio.com/fullsize2/ARC-NOL3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19561-img0005.jpg)
![Immunohistochemistry-Paraffin: ARC/NOL3 Antibody [NBP2-19561] - Analysis of Muscle.](http://images.novusbio.com/fullsize2/ARC-NOL3-Antibody-Immunohistochemistry-Paraffin-NBP2-19561-img0007.jpg)
![Western Blot: ARC/ARG3.1 Antibody [NBP1-56929] - Reccomended Titration: 0.2 - 1 ug/ml ELISA Titer: 1:312500 Positive Control: Transfected 293T](http://images.novusbio.com/fullsize2/ARC-ARG3.1 Antibody-Western Blot-NBP1-56929-img0010.jpg)
![Immunohistochemistry-Paraffin: ARC/ARG3.1 Antibody [NBP1-56929] - Human adrenal tissue at an antibody concentration of 5ug/ml.](http://images.novusbio.com/fullsize2/ARC-ARG3.1-Antibody-Immunohistochemistry-Paraffin-NBP1-56929-img0004.jpg)
![Western Blot: ARC/ARG3.1 Antibody [NBP1-56929] - Analysis of 293T cell lysate. Antibody Dilution: 1.0 ug/ml.](http://images.novusbio.com/fullsize2/ARC-ARG3.1-Antibody-Western-Blot-NBP1-56929-img0005.jpg)
![Western Blot: NOD2 Antibody (2D9) [NB100-524] - Total protein from human THP1 and US-OS cells and mouse Raw264.7 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-NOD2 in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/images2/NOD2-Antibody-(2D9)-Western-Blot-NB100-524-img0013.jpg "Western Blot: NOD2 Antibody (2D9) [NB100-524] - Total protein from human THP1 and US-OS cells and mouse Raw264.7 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-NOD2 in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence.")
