![SDS-Page: Rat IgM Isotype Control [HRP] [NBP1-97203] - Lane M: 3 uL Opal Prestained Marker. Lane 1: Reduced Rat IgG Whole Molecule. Lane 2: Reduced Rat IgG F(c) Fragment. Lane 3: Reduced Rat IgG Fab Fragment. Lane 4: Reduced Rat IgM Whole Molecule Peroxidase Conjugated. Load: 1 ug of IgG, F(c), Fab; 1.5 ug of IgM.](http://images.novusbio.com/fullsize2/Rat-IgM-Isotype-Control-[HRP]-SDS-Page-NBP1-97203-img0001.jpg "SDS-Page: Rat IgM Isotype Control [HRP] [NBP1-97203] - Lane M: 3 uL Opal Prestained Marker. Lane 1: Reduced Rat IgG Whole Molecule. Lane 2: Reduced Rat IgG F(c) Fragment. Lane 3: Reduced Rat IgG Fab Fragment. Lane 4: Reduced Rat IgM Whole Molecule Peroxidase Conjugated. Load: 1 ug of IgG, F(c), Fab; 1.5 ug of IgM.")
| Reactivity | RtSpecies Glossary |
| Applications | PAGE |
| Host | Rat |
| Conjugate | HRP |
| Concentration | LYOPH |
| Description | This Rat IgM immunoglobulin is useful as an isotype control for Rat IgM antibody. |
| Specificity | Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti- Rat IgM and anti-Rat Serum. |
| Isotype | IgM |
| Host | Rat |
| Gene | IGHM |
| Purity | Size Exclusion Chromatography |
| Dilutions |
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| Application Notes | NOTE: Do NOT add Sodium Azide (it inhibits HRP irreversibly). |
| Readout System |
| Storage | Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Lyophilized from 0.04 M Potassium Phosphate, 0.30 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free |
| Preservative | 0.01% Gentamicin Sulfate |
| Concentration | LYOPH |
| Purity | Size Exclusion Chromatography |
| Reconstitution Instructions | Reconstitute with 500 ul deionized water |
![Rat IgM Isotype Control [HRP]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "Rat IgM Isotype Control [HRP]")
Diseases for Rat IgM Isotype Control (NBP1-97203)Discover more about diseases related to Rat IgM Isotype Control (NBP1-97203).
| Pathways for Rat IgM Isotype Control (NBP1-97203)View related products by pathway.
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How to identify B cell subsets using flow cytometry By Victoria OsinskiUsing flow cytometry to identify B cell subsetsIdentifying cellular subsets by flow cytometry requires careful and thorough planning in order to ensure the correct subset of cells are identified a... Read full blog post. |
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Understanding the Importance of Human IgG Human IgG is a component of the immune system that protects the body from infection. It is the most abundantly found antibody isotype within the circulatory system of the human body. All antibody isotypes contain two heavy chains and two light chains... Read full blog post. |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | IGHM |
![Western Blot: MLC1 Antibody [NBP1-81555] - Analysis in control (vector only transfected HEK293T lysate) and MLC1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize2/MLC1-Antibody-Western-Blot-NBP1-81555-img0008.jpg)
![Immunohistochemistry-Paraffin: MLC1 Antibody [NBP1-81555] - Staining in human cerebral cortex and pancreas tissues using anti-MLC1 antibody. Corresponding MLC1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize2/MLC1-Antibody-Immunohistochemistry-Paraffin-NBP1-81555-img0007.jpg)
![Immunohistochemistry-Paraffin: MLC1 Antibody [NBP1-81555] - Staining of human cerebral cortex shows high expression.](http://images.novusbio.com/fullsize2/MLC1-Antibody-Immunohistochemistry-Paraffin-NBP1-81555-img0005.jpg)
![Western Blot: RUNX1T1/ETO Antibody [NBP2-55747] - H2135 and H2087 non-small cell lung cancer cells showing baseline Runx1T1 expression versus Runx1T1 stable overexpression. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize2/RUNX1T1-ETO-Antibody-Western-Blot-NBP2-55747-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: RUNX1T1/ETO Antibody [NBP2-55747] - Staining of human cell line HEK 293 shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize2/RUNX1T1-ETO-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-55747-img0001.jpg)
![Western Blot: Aldo-keto Reductase 1C4/AKR1C4 Antibody (2C11) [H00001109-M01] - AKR1C4 monoclonal antibody (M01), clone 2C11 Analysis of AKR1C4 expression in MCF-7.](http://images.novusbio.com/fullsize2/Aldo-keto-Reductase-1C4-AKR1C4-Antibody-(2C11)-Western-Blot-H00001109-M01-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: Aldo-keto Reductase 1C4/AKR1C4 Antibody (2C11) [H00001109-M01] - Analysis of monoclonal antibody to AKR1C4 on HepG2 cell. Antibody concentration 15 ug/ml.](http://images.novusbio.com/fullsize2/Aldo-keto-Reductase-1C4-AKR1C4-Antibody-(2C11)-Immunocytochemistry-Immunofluorescence-H00001109-M01-img0011.jpg)
![Immunohistochemistry-Paraffin: Aldo-keto Reductase 1C4/AKR1C4 Antibody (2C11) [H00001109-M01] - Analysis of monoclonal antibody to AKR1C4 on formalin-fixed paraffin-embedded human liver tissue. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize2/Aldo-keto-Reductase-1C4-AKR1C4-Antibody-(2C11)-Immunohistochemistry-Paraffin-H00001109-M01-img0013.jpg)
![Western Blot: beta-Galactosidase-1/GLB1 Antibody (1C9) [NBP2-45731] - Analysis of extracts (35ug) from 9 different cell lines by using anti-GLB1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize2/beta-Galactosidase-1-GLB1-Antibody-(1C9)-Western-Blot-NBP2-45731-img0016.jpg)
![Immunohistochemistry: beta-Galactosidase-1/GLB1 Antibody (1C9) [NBP2-45731] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize2/beta-Galactosidase-1-GLB1-Antibody-(1C9)-Immunohistochemistry-NBP2-45731-img0013.jpg)
![Western Blot: beta-Galactosidase-1/GLB1 Antibody (1C9) [NBP2-45731] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GLB1.](http://images.novusbio.com/fullsize2/beta-Galactosidase-1-GLB1-Antibody-(1C9)-Western-Blot-NBP2-45731-img0014.jpg)
![Western Blot: IFN-alpha 1 Antibody [NBP2-75930] - analysis of IFNA1 on mouse liver tissue lysate using anti-IFNA1 antibody at 1/100 dilution.](http://images.novusbio.com/fullsize2/IFN-alpha-1-Antibody-Western-Blot-NBP2-75930-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: IFN-alpha 1 Antibody [NBP2-75930] - staining IFNA1 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize2/IFN-alpha-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-75930-img0004.jpg)
![Immunohistochemistry-Paraffin: IFN-alpha 1 Antibody [NBP2-75930] - analysis of paraffin-embedded mouse brain tissue tissue using anti-IFNA1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize2/IFN-alpha-1-Antibody-Immunohistochemistry-Paraffin-NBP2-75930-img0007.jpg)
![Western Blot: CDR2 Antibody (33) [NBP2-10509] - Western blot analysis of CDR2 expression in 1) HeLa control cell lysate and 2) HeLa cell lysate overexpressing CDR2.](http://images.novusbio.com/fullsize/CDR2-Antibody-(33)-Western-Blot-NBP2-10509-img0003.jpg)
![Immunohistochemistry: CDR2 Antibody (33) [NBP2-10509] - IHC staining of CDR2 in human breast cancer using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize2/CDR2-Antibody-(33)-Immunohistochemistry-NBP2-10509-img0004.jpg)
![Western Blot: CDR2 Antibody (33) [NBP2-10509] - Western blot analysis of CDR2 expression in 1) HeLa, 2) NTERA-2 and 3) HepG2 whole cell lysates.](http://images.novusbio.com/fullsize/CDR2-Antibody-(33)-Western-Blot-NBP2-10509-img0002.jpg)
![Western Blot: Glutathione S Transferase kappa 1 Antibody [NBP2-16686] - Sample (30 ug of whole cell lysate) A: Hep G2 12% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize2/Glutathione S Transferase kappa 1 Antibody-Western Blot-NBP2-16686-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Glutathione S Transferase kappa 1 Antibody [NBP2-16686] - Immunofluorescence analysis of methanol-fixed A549, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize2/Glutathione S Transferase kappa 1 Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16686-img0003.jpg)
![Immunohistochemistry-Paraffin: Glutathione S Transferase kappa 1 Antibody [NBP2-16686] - Immunohistochemical analysis of paraffin-embedded gastric ca, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize2/Glutathione S Transferase kappa 1 Antibody-Immunohistochemistry-Paraffin-NBP2-16686-img0002.jpg)
![Immunohistochemistry-Paraffin: SLCO6A1 Antibody [NBP2-49118] - Staining of human testis shows high expression.](http://images.novusbio.com/fullsize2/SLCO6A1-Antibody-Immunohistochemistry-Paraffin-NBP2-49118-img0004.jpg)
![Immunohistochemistry-Paraffin: SLCO6A1 Antibody [NBP2-49118] - Staining of human endometrium shows low expression as expected.](http://images.novusbio.com/fullsize2/SLCO6A1-Antibody-Immunohistochemistry-Paraffin-NBP2-49118-img0003.jpg)
![Immunohistochemistry-Paraffin: SLCO6A1 Antibody [NBP2-49118] - Staining in human testis and endometrium tissues using anti-SLCO6A1 antibody. Corresponding SLCO6A1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize2/SLCO6A1 Antibody-Immunohistochemistry-Paraffin-NBP2-49118-img0005.jpg)
![Western Blot: MKP-1/DUSP1 Antibody (JJ0930) [NBP2-67909] - Analysis of DUSP1 on mouse spleen lysates using anti-DUSP1 antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize2/MKP-1-DUSP1 Antibody (JJ0930)-Western Blot-NBP2-67909-img0004.jpg)
![Immunohistochemistry-Paraffin: MKP-1/DUSP1 Antibody (JJ0930) [NBP2-67909] - Analysis of paraffin-embedded mouse lung tissue using anti-DUSP1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize2/MKP-1-DUSP1-Antibody-(JJ0930)-Immunohistochemistry-Paraffin-NBP2-67909-img0003.jpg)
![Flow Cytometry: MKP-1/DUSP1 Antibody (JJ0930) [NBP2-67909] - Analysis of HepG2 cells with DUSP1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody](http://images.novusbio.com/fullsize2/MKP-1-DUSP1-Antibody-(JJ0930)-Flow-Cytometry-NBP2-67909-img0001.jpg)
![Flow Cytometry: HLA DQ Antibody (SPV-L3) [NBP2-45041] - Flow Cytometric Analysis of Raji cells. HLA DQ Antibody (SPV-L3) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize2/HLA DQ Antibody (SPV-L3)-Flow Cytometry-NBP2-45041-img0001.jpg)
![Western Blot: NOD2 Antibody (2D9) [NB100-524] - Total protein from human THP1 and US-OS cells and mouse Raw264.7 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-NOD2 in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize2/NOD2-Antibody-(2D9)-Western-Blot-NB100-524-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: NOD2 Antibody (2D9) [NB100-524] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-NOD2 (2D9) NB100-524 at a 1:200 dilution overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Actin was counterstained with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize2/NOD2-Antibody-(2D9)-Immunocytochemistry-Immunofluorescence-NB100-524-img0008.jpg)
![Immunohistochemistry-Frozen: NOD2 Antibody (2D9) [NB100-524] - Overlay of NOD2-DyLight 488 (green) with phase contrast of murine colon. Image from verified customer review.](http://images.novusbio.com/fullsize2/NOD2-Antibody-(2D9)-Immunohistochemistry-Frozen-NB100-524-img0016.jpg)
![Western Blot: Complement C4b/d Antibody (16D2) [NB200-541] - Western blot analysis of C4 expression in J774.A1 (A) aand mouse PBL (B) whole cell lysates.](http://images.novusbio.com/fullsize2/Complement-C4b-d-Antibody-(16D2)-Western-Blot-NB200-541-img0001.jpg)
![Western Blot: ARC/NOL3 Antibody [NBP2-19561] - Sample (30 ug of whole cell lysate) A: HeLa 12% SDS PAGE diluted at 1:1000](http://images.novusbio.com/fullsize2/ARC-NOL3-Antibody-Western-Blot-NBP2-19561-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: ARC/NOL3 Antibody [NBP2-19561] - Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using NOL3 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.](http://images.novusbio.com/fullsize2/ARC-NOL3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19561-img0005.jpg)
![Immunohistochemistry-Paraffin: ARC/NOL3 Antibody [NBP2-19561] - Analysis of Muscle.](http://images.novusbio.com/fullsize2/ARC-NOL3-Antibody-Immunohistochemistry-Paraffin-NBP2-19561-img0007.jpg)
![Western Blot: ARC/ARG3.1 Antibody [NBP1-56929] - Reccomended Titration: 0.2 - 1 ug/ml ELISA Titer: 1:312500 Positive Control: Transfected 293T](http://images.novusbio.com/fullsize2/ARC-ARG3.1 Antibody-Western Blot-NBP1-56929-img0010.jpg)
![Immunohistochemistry-Paraffin: ARC/ARG3.1 Antibody [NBP1-56929] - Human adrenal tissue at an antibody concentration of 5ug/ml.](http://images.novusbio.com/fullsize2/ARC-ARG3.1-Antibody-Immunohistochemistry-Paraffin-NBP1-56929-img0004.jpg)
![Western Blot: ARC/ARG3.1 Antibody [NBP1-56929] - Analysis of 293T cell lysate. Antibody Dilution: 1.0 ug/ml.](http://images.novusbio.com/fullsize2/ARC-ARG3.1-Antibody-Western-Blot-NBP1-56929-img0005.jpg)
![Western Blot: NOD2 Antibody (2D9) [NB100-524] - Total protein from human THP1 and US-OS cells and mouse Raw264.7 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-NOD2 in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/images2/NOD2-Antibody-(2D9)-Western-Blot-NB100-524-img0013.jpg "Western Blot: NOD2 Antibody (2D9) [NB100-524] - Total protein from human THP1 and US-OS cells and mouse Raw264.7 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-NOD2 in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence.")
