Sheep IgM Isotype Control

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SDS-Page: Sheep IgM Isotype Control [NBP1-96774] - Lane 1: Sheep IgM, Non-Reduced. Lane 2: Sheep IgM, Reduced. Load: 1.0 ug per lane.

Product Details

Summary
Reactivity ShSpecies Glossary
Applications WB, ELISA, IHC, IHC-Fr, IHC-P, IP, PAGE
Host
Sheep
Conjugate
Unconjugated
Format
Purified
Concentration
4.48 mg/ml

Order Details

Sheep IgM Isotype Control Summary

Description
This Sheep IgM immunoglobulin is useful as an isotype control for Sheep IgM antibody.
Specificity
Sheep IgM whole molecule was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Sheep Serum and anti-Sheep IgM (mu chain specific). No reaction was observed against anti-Sheep IgG F(c). Some light chain cross reactivity will occur with anti-Sheep IgG.
Isotype
IgM
Host
Sheep
Purity
Size Exclusion Chromatography

Applications/Dilutions

Dilutions
  • Western Blot 1:100 - 1:2000
  • ELISA 1:100 - 1:2000
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:10 - 1:500
  • Immunoprecipitation 1:10 - 1:500
  • SDS-Page 1:100 - 1:2000

Packaging, Storage & Formulations

Storage
Store at 4C short term. For extended storage, add an equal volume of glycerol, aliquot and store at -20C or below. Avoid repeated freeze-thaw cycles.
Buffer
0.1 M Tris Chloride, 0.5 M Sodium Chloride, pH 8.0
Preservative
0.1% Sodium Azide
Concentration
4.48 mg/ml
Purity
Size Exclusion Chromatography

Alternate Names for Sheep IgM Isotype Control

  • AGM1
  • DKFZp686I15196
  • DKFZp686I15212
  • FLJ00385
  • IgM
  • immunoglobulin heavy constant mu
  • MGC104996
  • MGC52291
  • MU
  • VH

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

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Human IgG is a component of the immune system that protects the body from infection. It is the most abundantly found antibody isotype within the circulatory system of the human body. All antibody isotypes contain two heavy chains and two light chains...  Read full blog post.

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