Mouse IgM Isotype Control (F8)

Product Discontinued

Mouse IgM Isotype Control (F8) Summary

• Description: This Mouse IgM (F8) immunoglobulin is useful as an isotype control for Mouse IgM (F8) antibody. This antibody is purified from culture supernatant of a hybridoma (Mouse myeloma fused with spleen cells from a Mouse, clone F8) with protein A/G beads.
• Isotype: IgM
• Clonality: Monoclonal
• Host: Mouse
• Gene: IGHM
• Purity: Protein L purified

Applications/Dilutions

• Dilutions:
  • Block/Neutralize
  • Functional
  • In vivo assay
• Application Notes: Use the isotype control at the same concentration as the experimental antibody. Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS. 250 ug size is provided in liquid form, PBS
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein L purified
• Reconstitution Instructions: Reconstitute with PBS.

Alternate Names for Mouse IgM Isotype Control (F8)

• AGM1
• DKFZp686I15196
• DKFZp686I15212
• FLJ00385
• IgM
• immunoglobulin heavy constant mu
• MGC104996
• MGC52291
• MU
• VH

Background

IgM normally constitutes about 10% of serum immunoglobulins. IgM antibody is prominent in early immune responses to most antigens and is largely confined to plasma due to its large size. Monomeric IgM is expressed as a membrane bound antibody on the surface of B cells and as a pentamer when secreted by plasma cells. Due to its high valency IgM is more efficient than other isotypes is binding antigens with repeating epitopes (virus particles and red blood cells) and is more efficient than IgG in activiating the complement pathway. The gene for the mu constant region contains four domains separated by short intervening sequences. IgM measurement yields information about the body's immediate resistance and response to infection as well as information related to specific diseases. Decreased levels are associated with immune deficiency states, hereditary deficiencies, and myeloma. Increased levels can be associated with Waldenstrom's macroglobulinemia, chronic infection and hepatocellular disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

Publications for IgM Isotype Control (NBP2-21945)(2)

Publications using NBP2-21945

• Wang X, He Q, Zhou C et al. Prolonged hypernutrition impairs TREM2-dependent efferocytosis to license chronic liver inflammation and NASH development Immunity 2022-12-09 [PMID: 36521495]
• Venugopal S, Chen M, Liao W et al. Isthmin is a novel vascular permeability inducer that functions through cell-surface GRP78-mediated Src activation. Cardiovasc. Res. 2015-05-06 [PMID: 25952901] (In Vivo, Func, B/N, Human)

Bioinformatics

• Gene Symbol: IGHM