Rabbit anti-Sheep IgM Heavy Chain Secondary Antibody [FITC]

Product Discontinued

Rabbit anti-Sheep IgM Heavy Chain Secondary Antibody [FITC] Summary

• Description: This antibody is purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
• Immunogen: Sheep IgM mu heavy chain
• Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Sheep IgM and Sheep Serum. No reaction was observed against Sheep IgG.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: IGHM
• Purity: Ion exchange chromatography

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:1:10 - 1:1000
  • Fluorophore-linked immunosorbent assay 1:10000 - 1:50000
  • Immunocytochemistry/ Immunofluorescence 1:1000 - 1:5000

Reactivity Notes

Reacts with Sheep.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
• Preservative: 0.01% Sodium Azide
• Purity: Ion exchange chromatography

Notes

Emission Wavelength: 528, Excitation Wavelength: 495, Label Molecular Weight: 390, Restore with 2.0 mL of deionized water (or equivalent).

Alternate Names for Rabbit anti-Sheep IgM Heavy Chain Secondary Antibody [FITC]

• Constant region of heavy chain of IgM
• Ig Mu Chain C Region BOT
• Ig Mu Chain C Region GAL
• Ig Mu Chain C Region OU
• immunoglobulin heavy constant mu
• Immunoglobulin mu chain

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IGHM