Rabbit anti-Canine IgM Heavy Chain Secondary Antibody [HRP]

Dot Blot: Rabbit anti-Canine IgM Heavy Chain Secondary Antibody [HRP] [NBP1-73847] - Dot Blot results of Rabbit anti-Canine IgM Heavy Chain Secondary antibody [HRP].Dots are Dog IgM at (1) 100ng, (2) 33.3ng, (3) 11.1ng, (4) 3.70ng, (5) 1.23ng. Blocking: 30 min at RT.Primary Antibody: none.Secondary Antibody: Rabbit Anti-Dog IgM -HRP at 1ug/mL for 1hr at RT.

Dot Blot results of Rabbit Anti-Dog IgM Antibody Peroxidase Conjugated. Dots are Dog IgM at (1) 100ng, (2) 33.3ng, (3) 11.1ng, (4) 3.70ng, (5) 1.23ng. Blocking: for 30 min at RT. Primary Antibody: none. Secondary Antibody: Rabbit Anti-Dog IgM-HRP at 1ug/mL for 1hr at RT. Imaged with BioRad ChemiDoc, Chemi filter.

• Reactivity: Ca
• Applications: WB, DB, ELISA, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: HRP

Rabbit anti-Canine IgM Heavy Chain Secondary Antibody [HRP] Summary

• Description: For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room
  
  This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Dog IgM and Dog Serum
• Immunogen: Dog IgM mu heavy chain
• Specificity: No reaction was observed against Dog IgG.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: IGHM
• Purity: Multi-step

Applications/Dilutions

• Dilutions:
  • Dot Blot
  • ELISA 1:10000 - 1:50000
  • Immunohistochemistry 1:500 - 1:2500
  • Western Blot 1:1000 - 1:10000
• Application Notes: This product has been tested dot blot and ELISA. This product is assayed against 1.0 ug of Dog IgM in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.
  
  NOTE: Do NOT add Sodium Azide (it inhibits HRP irreversibly).
• Readout System:
  • NovaLume Plus Chemiluminescent Reagent LUM-555

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
• Preservative: 0.01% Gentamicin Sulfate
• Purity: Multi-step

Alternate Names for Rabbit anti-Canine IgM Heavy Chain Secondary Antibody [HRP]

• Constant region of heavy chain of IgM
• Ig Mu Chain C Region BOT
• Ig Mu Chain C Region GAL
• Ig Mu Chain C Region OU
• immunoglobulin heavy constant mu
• Immunoglobulin mu chain

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IGHM