Rabbit anti-Feline IgM Heavy Chain Secondary Antibody [FITC]

Flow Cytometry: Rabbit anti-Feline IgM Heavy Chain Secondary Antibody [FITC] [NBP1-73836] - Phenotyping of cat blood. Cat blood cells were washed and incubated with the antibody. It was used as a secondary antibody to check for bound IgM, in cat blood. It served as a B cell marker. This image was submitted via customer review.

• Reactivity: Fe
• Applications: ELISA, Flow, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: FITC

Rabbit anti-Feline IgM Heavy Chain Secondary Antibody [FITC] Summary

• Description: For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room
  
  This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-Rabbit Serum, Cat IgM and Cat Serum
• Immunogen: Cat IgM mu heavy chain
• Specificity: No reaction was observed against Cat IgG.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: IGHM
• Purity: Multi-step

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:1:10 - 1:1000
  • Fluorophore-linked immunosorbent assay 1:10000 - 1:50000
  • Immunocytochemistry/ Immunofluorescence 1:1000 - 1:5000
• Application Notes: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
• Preservative: 0.01% Sodium Azide
• Purity: Multi-step

Alternate Names for Rabbit anti-Feline IgM Heavy Chain Secondary Antibody [FITC]

• Constant region of heavy chain of IgM
• Ig Mu Chain C Region BOT
• Ig Mu Chain C Region GAL
• Ig Mu Chain C Region OU
• immunoglobulin heavy constant mu
• Immunoglobulin mu chain

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Review for IgM Heavy Chain Antibody (NBP1-73836) (1)

Average Rating: 3

(Based on 1 review)

We have 1 review tested in 1 species: Feline.

reviewed by: Verified Customer Flow Feline 02/01/2018

Summary

• Application: Flow Cytometry
• Sample Tested: Whole blood cells
• Species: Feline
• Lot: 4131

Comments

• Comments: Cat blood cells were washed and incubated with the antibody. It was used as a secondary antibody to check for bound IgM, in cat blood. It served as a B cell marker.

Bioinformatics

• Gene Symbol: IGHM