Rabbit anti-Porcine IgM Heavy Chain Secondary Antibody

• Reactivity: Po
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: LYOPH

Rabbit anti-Porcine IgM Heavy Chain Secondary Antibody Summary

• Description: This product was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, Swine IgM and Swine Serum
  
  Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
• Immunogen: Swine IgM mu heavy chain
• Specificity: No reaction was observed against Swine IgG.
• Isotype: Serum
• Clonality: Polyclonal
• Host: Rabbit
• Gene: IGHM
• Purity: Delipidation and Defibrination

Applications/Dilutions

• Dilutions:
  • ELISA 1:20000 - 1:100000
  • Immunohistochemistry 1:1000 - 1:5000
  • Western Blot 1:2000 - 1:10000
• Application Notes: This product are ideal for ELISA, western blotting, Immunohistochemistry, as well as other antibody detection methods.

Packaging, Storage & Formulations

• Storage: Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Concentration: LYOPH
• Purity: Delipidation and Defibrination
• Reconstitution Instructions: Reconstitute with 2.0 ml deionized water (or equivalent).

Alternate Names for Rabbit anti-Porcine IgM Heavy Chain Secondary Antibody

• Constant region of heavy chain of IgM
• Ig Mu Chain C Region BOT
• Ig Mu Chain C Region GAL
• Ig Mu Chain C Region OU
• immunoglobulin heavy constant mu
• Immunoglobulin mu chain

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IgM Antibody (NBP1-73112)(2)

Publications using NBP1-73112

• Wiarda J, Shircliff A, Stasko J et al. Conserved B cell signaling, activation, and differentiation in porcine jejunal and ileal Peyer's patches despite distinct immune landscapes bioRxiv 2023-09-15 (IHC)
• Wiarda JE, Shircliff AL, Becker SR et Al. Conserved B cell signaling, activation, and differentiation in porcine jejunal and ileal Peyer's patches despite distinct immune landscapes Mucosal Immunol 2024-08-13 [PMID: 39147277]

Bioinformatics

• Gene Symbol: IGHM