Goat F(ab')2 anti-Mouse IgM Heavy Chain Secondary Antibody [HRP]

ELISA: Goat F(ab')2 anti-Mouse IgM Heavy Chain Secondary Antibody [HRP] [NB120-5930] - ELISA Results of Goat F(ab')2 anti-Mouse IgM Heavy Chain Secondary antibody [HRP]. Each well was coated in duplicate with 1.0 ug of Mouse IgM . The working dilution is 115,000. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using TMB substrate.

• Reactivity: Mu
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: HRP

Goat F(ab')2 anti-Mouse IgM Heavy Chain Secondary Antibody [HRP] Summary

• Description: For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room
  
  This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Mouse IgM and Mouse Serum
• Immunogen: Goat F(ab')2 anti-Mouse IgM Heavy Chain Secondary Antibody was produced by repeated immunization with Mouse IgM heavy chain in goat.
• Specificity: No reaction was observed against anti-Pepsin or anti-Goat IgG F(c). No reaction was observed against other mouse heavy or light chain proteins.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IGHM
• Purity: Multi-step

Applications/Dilutions

• Dilutions:
  • ELISA 1:25000 - 1:50000
  • Immunohistochemistry 1:500 - 1:2500
  • Western Blot 1:2000 - 1:10000
• Application Notes: This product has been tested by ELISA and is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user. NOTE: Do NOT add Sodium Azide (it inhibits HRP irreversibly).
• Readout System:
  • NovaLume Plus Chemiluminescent Reagent LUM-555

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
• Preservative: 0.01% Gentamicin Sulfate
• Purity: Multi-step

Alternate Names for Goat F(ab')2 anti-Mouse IgM Heavy Chain Secondary Antibody [HRP]

• Constant region of heavy chain of IgM
• Ig Mu Chain C Region BOT
• Ig Mu Chain C Region GAL
• Ig Mu Chain C Region OU
• immunoglobulin heavy constant mu
• Immunoglobulin mu chain

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IGHM