Mouse IgM ELISA Kit (Colorimetric)

ELISA: IgM ELISA Kit (Colorimetric) [NBP2-61294] - ENZO ELISA Project

• Reactivity: Mu
• Applications: ELISA
• Suitable Sample Type: Ascites fluid, culture supernatants, and serum of mouse origin
• Standard Curve Range: 3.91 - 250 ng/ml
• Sensitivity: 0.60 ng/ml

Mouse IgM ELISA Kit (Colorimetric) Summary

• Standard Curve Range: 3.91 - 250 ng/ml
• Sensitivity: 0.60 ng/ml
• Assay Type: Competitive ELISA
• Inter-Assay: Mean 6.4%
• Intra-Assay: Mean 3.9%
• Spike Recovery: Mouse Serum - 102.8%
  Tissue Culture Media - 92.9%
  Mouse Ascites Fluid - 94.6%
• Sample Volume: 50 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: IGHM

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Store at 4°C.

Alternate Names for Mouse IgM ELISA Kit (Colorimetric)

• AGM1
• DKFZp686I15196
• DKFZp686I15212
• FLJ00385
• IgM
• immunoglobulin heavy constant mu
• MGC104996
• MGC52291
• MU
• VH

Background

Immunoglobulin M (IgM) is an immunoglobulin expressed initially on the surface of B-cells as a monomer of two mu heavy chains and two light chains. Following B-cell activation, IgM is secreted in its pentameric form, consisting of five IgM molecules joined at the base of the Fc domain by a J chain. IgM is the first Immunoglobulin expressed following infection.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for IgM ELISA Kit (NBP2-61294)(1)

Publication using NBP2-61294

• Xu X, Sui B, Liu X, Sun J Superior low-immunogenicity of tilapia type I collagen based on unique secondary structure with single calcium binding motif over terrestrial mammals by inhibiting activation of DC intracellular Ca2+-mediated STIM1-Orai1/NF-kappa B pathway Materials Science and Engineering: C 2021-01-01 [PMID: 34857289] (ELISA)

FAQs for IgM ELISA Kit (NBP2-61294). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: IGHM