Turkey IgM ELISA Kit (Colorimetric) Summary
| Description |
Quality control test: Standard curve. IgM (Turkey) ELISA Kit is for the quantitative measurement of IgM in plasma, serum and egg-yolk extracts. |
| Standard Curve Range |
3.13 to 200 ng/mL |
| Sensitivity |
3.13 to 200 ng/mL |
| Assay Type |
Quantification |
| Sample Volume |
100 uL |
| Kit Type |
ELISA Kit (Colorimetric) |
| Gene |
IGHM |
Applications/Dilutions
Packaging, Storage & Formulations
Kit Components
|
Components
|
- Anti Turkey IgM coated plate 96 wells (12 Strips of 8 wells), HRP Conjugate Reagent 11 mL, Reference Standard (lyophilized) 1 vial, 20x Wash Solution 50 mL, 10x Immunoglobulin Diluent 25 mL, TMB Reagent (One-step) 11mL, Stop Solution (1 N HCI) 11 mL
|
Notes
This product is produced by and distributed for Abnova, a company based in Taiwan.
Alternate Names for Turkey IgM ELISA Kit (Colorimetric)
Background
IgM normally constitutes about 10% of serum immunoglobulins. IgM antibody is prominent in early immune responses to most antigens and is largely confined to plasma due to its large size. Monomeric IgM is expressed as a membrane bound antibody on the surface of B cells and as a pentamer when secreted by plasma cells. Due to its high valency IgM is more efficient than other isotypes is binding antigens with repeating epitopes (virus particles and red blood cells) and is more efficient than IgG in activiating the complement pathway. The gene for the mu constant region contains four domains separated by short intervening sequences. IgM measurement yields information about the body's immediate resistance and response to infection as well as information related to specific diseases. Decreased levels are associated with immune deficiency states, hereditary deficiencies, and myeloma. Increased levels can be associated with Waldenstrom's macroglobulinemia, chronic infection and hepatocellular disease.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Product General Protocols
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FAQs for IgM ELISA Kit (KA2516). (Showing 1 - 1 of 1 FAQ).
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What is the difference between the Quantikine and Duoset ELISA kits?
- Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.