Goat anti-Mouse IgM Secondary Antibody [Allophycocyanin]

BG01V human embryonic stem cells were stained with MouseAnti-Human TRA-1-81 Monoclonal Antibody (Catalog # MAB8495,filled histogram) or mouse IgM isotype control antibody (open histogram),followed by Allophycocyanin-conjugated Anti-Mouse IgM Secondary Antibody (Catalog #F0117) at 10 μL/106 cells.

• Reactivity: Mu
• Applications: Flow
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Allophycocyanin

Goat anti-Mouse IgM Secondary Antibody [Allophycocyanin] Summary

• Immunogen: Mouse IgM
• Specificity: Mouse IgM specific IgG was purified by goat IgG affinity chromatography and then adsorbed to eliminate human cross reactivity. The IgG fraction was digested with pepsin to generate F(ab')₂ fragments which have a reduced ability to interact with Fc receptors expressed on a variety of cells.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IGHM
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Goat anti-Mouse IgM Secondary Antibody [Allophycocyanin]

• AGM1
• DKFZp686I15196
• DKFZp686I15212
• FLJ00385
• IgM
• immunoglobulin heavy constant mu
• MGC104996
• MGC52291
• MU
• VH

Background

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IgM Antibody (F0117)(1)

Publication using F0117

• C Liu, Q Gong, T Chen, J Lv, Z Feng, P Liu, Z Deng Treatment with 20(S)-ginsenoside Rg3 reverses multidrug resistance in A549/DDP xenograft tumors Oncol Lett, 2018-01-24;15(4):4376-4382. 2018-01-24 [PMID: 29541206] (Flow Cytometry, Mouse)

Bioinformatics

• Gene Symbol: IGHM