IgM Antibody (II/41) [FITC]

Images

 
Flow Cytometry: Mouse IgM Antibody (II/41) [FITC] [NBP1-43947] - Staining of C57BL/6 bone marrow cells with Anti-Human/Mouse CD45R (B220) APC and 0.125 ug of Rat IgG2a K Isotype Control FITC (left) or 0.125 ug of ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow
Clone
II/41
Clonality
Monoclonal
Host
Rat
Conjugate
FITC
Concentration
0.5 mg/ml

IgM Antibody (II/41) [FITC] Summary

Immunogen
The immunogen for this antibody was IgM.
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Rat
Gene
IGHM
Purity
Protein A or G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10-1:1000
Application Notes
The II/41 antibody has been tested by flow cytometric analysis of mouse spleen cell suspensions. This can be used at less than or equal to 0.125 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test.

Reactivity Notes

This antibody is reactive to Mouse.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS (pH 7.2) with 0.1% gelatin
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Alternate Names for IgM Antibody (II/41) [FITC]

  • AGM1
  • DKFZp686I15196
  • DKFZp686I15212
  • FLJ00385
  • IgM
  • immunoglobulin heavy constant mu
  • MGC104996
  • MGC52291
  • MU
  • VH

Background

The II/41 monoclonal antibody reacts with the mu heavy chain of mouse IgM. It does not react with other classes of mouse immunoglobulin including IgD, IgG or IgA. IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells. Fluorochrome conjugated II/41 can be used as a detection secondary for mouse IgM.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol IGHM