IFN-alpha Antibody [Unconjugated]

• Reactivity: Hu
• Applications: B/N
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

IFN-alpha Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human IFN-alpha
• Specificity: Human IFN-alpha
• Source: N/A
• Isotype: Serum
• Clonality: Polyclonal
• Host: Rabbit
• Gene: IFNA2
• Purity Statement: N/A

Applications/Dilutions

• Dilutions:
  • Neutralization

Packaging, Storage & Formulations

• Storage: After receipt, the product may be stored at -20°C for short-term use (≤ 6 months). For
  long-term storage, we recommend storing the product at -70°C or below for retention of full activity. When thawing, the
  contents of the tube should be apportioned in separate tubes so that freezing and thawing is kept to a minimum.
  Refreezing should be done on dry ice or in a dry ice/alcohol bath. Further dilution of the product should be in buffers
  containing protein such as 0.1% bovine serum albumin (BSA).
• Preservative: No Preservative

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IFN-alpha Antibody [Unconjugated]

• IFNA
• IFNA2
• IFNA2b
• IFNalpha
• IFN-alpha
• IFN-alphaA
• INFA2
• interferon alpha 2
• LeIF D

Background

The most common use of this product has been in a pool with Rabbit Anti-Human Interferon Beta (PBL # 31410) and Mouse Anti-IFNAR2 (PBL# 21385) to block the activity of all Type I IFNs. Krug et al [Eur. J. Immunol. (2001) 31:2154] used a pool of PBL #31101, #31410 and #21385 to demonstrate PBMC-derived Type I IFN is responsible for increased IFN-gamma secretion after stimulation with CpG ODN. Ito et al. [J. Exp. Med. (2002) 195:1507] used a similar pool to determine that TLR7 stimulated pDC but not mDC induce Th1 development through Type I IFN. Lande et al. [J. Immunol. (2003) 170:1174] used a pool of PBL#31410 and #31101 to show a blockade of autocrine Alpha/Beta action leads to decreased CXCL-10 secretion from Mycobacterium-infected dendritic cells. Jego et al. [Immunity (2003) 19:225] demonstrated the inhibition of IFN-alpha signaling in a co-culture of pDCs with CD40 stimulated B-cells reduces IgG secretion, while inhibition of IFN-beta signaling does not occur. Ku et al. [J. Exp. Med. (2004) 200:917] showed epithelial cells surrounding Varicella Zoster lesions express IFN-alpha by immunohistochemistry (IHC) in a SCID-human skin zenograft model.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IFN-alpha Antibody (31101-1)(2)

Publications using 31101-1

• Vanessa M.A. Woods, Lisette J. Latorre-Rey, Franziska Schenk, Marcel G.E. Rommel, Thomas Moritz, Ute Modlich Targeting transgenic proteins to alpha granules for platelet-directed gene therapy Molecular Therapy - Nucleic Acids 2022-03-08 [PMID: 35116189]
• Richez C, Yasuda K, Watkins AA TLR4 ligands induce IFN-alpha production by mouse conventional dendritic cells and human monocytes after IFN-beta priming. J. Immunol., 2009-01-15;182(2):820-8. 2009-01-15 [PMID: 19124725] (ELISA Development, Mouse)

Bioinformatics

• Gene Symbol: IFNA2