IFN-alpha Antibody (F17) [Unconjugated]

• Reactivity: Po
• Applications: B/N
• Clone: F17
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

IFN-alpha Antibody (F17) [Unconjugated] Summary

• Immunogen: Porcine IFN-alpha
• Specificity: Neutralizes pig interferon alpha
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant
• Endotoxin Note: <1.0 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Neutralization

Packaging, Storage & Formulations

• Storage: After receipt, this product should be kept at -20°C for retention of full activity. Do not store below -20°C.
• Buffer: Supplied as a liquid
• Preservative: No Preservative

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IFN-alpha Antibody (F17) [Unconjugated]

• IFNA
• IFNA2
• IFNA2b
• IFNalpha
• IFN-alpha
• IFN-alphaA
• INFA2
• interferon alpha 2
• LeIF D

Background

Interferon-alpha (IFN-alpha), also known as leukocyte interferon, represents a group of related but distinct proteins that share over 95% amino acid sequence homology. They are members of the type I interferon family which share a common cell surface receptor composed of two subunits, a 100 kDa ligand-binding subunit (IFN-alpha R2) and a 125 kDa ligand binding and signal transduction subunit (IFN-alpha R1) that is involved both in ligand binding and signal transduction. IFN-alpha has both anti-viral and immunomodulatory activities on target cells.

1. L'Haridon, R.M. et al. 1991, Hybridoma 10: 35-47.
2. Splichal, I. et al. 1994, Immunology Letters 43:203-208.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IFN-alpha Antibody (27105-1)(9)

Publications using 27105-1

• J Chepngeno, S Takanashi, A Diaz, H Michael, FC Paim, MC Rahe, JR Hayes, C Baker, D Marthaler, LJ Saif, AN Vlasova Comparative Sequence Analysis of Historic and Current Porcine Rotavirus C Strains and Their Pathogenesis in 3-Day-Old and 3-Week-Old Piglets Front Microbiol, 2020-04-24;11(0):780. 2020-04-24 [PMID: 32395116] (ELISA Detection, Porcine)
• Baumann A, Demoulins T, Python S, Summerfield A Porcine cathelicidins efficiently complex and deliver nucleic acids to plasmacytoid dendritic cells and can thereby mediate bacteria-induced IFN-alpha responses. J Immunol, 2014-06-04;193(1):364-71. 2014-06-04 [PMID: 24899499] (Flow Cytometry, Porcine)
• Ana M. González, Marli S. P. Azevedo, Kwonil Jung, Anastasia Vlasova, Wei Zhang, Linda J. Saif Innate immune responses to human rotavirus in the neonatal gnotobiotic piglet disease model Immunology 2010-10-01 [PMID: 20497255]
• Miller LC, Zanella EL, Waters WR Cytokine protein expression levels in tracheobronchial lymph node homogenates of pigs infected with pseudorabies virus. Clin. Vaccine Immunol., 2010-03-10;17(5):728-34. 2010-03-10 [PMID: 20219878] (ELISA Development, Porcine)
• de Los Santos T, de Avila Botton S, Weiblen R, Grubman MJ The leader proteinase of foot-and-mouth disease virus inhibits the induction of beta interferon mRNA and blocks the host innate immune response. J. Virol., 2006-02-01;80(4):1906-14. 2006-02-01 [PMID: 16439546] (ELISA Development, Porcine)
• Balmelli C, Vincent IE, Rau H, Guzylack-Piriou L, McCullough K, Summerfield A Fc gamma RII-dependent sensitisation of natural interferon-producing cells for viral infection and interferon-alpha responses. Eur. J. Immunol., 2005-08-01;35(8):2406-15. 2005-08-01 [PMID: 16021600] (ELISA Development, Porcine)
• Lee SM, Schommer SK, Kleiboeker SB Porcine reproductive and respiratory syndrome virus field isolates differ in in vitro interferon phenotypes. Vet. Immunol. Immunopathol., 2004-12-08;102(3):217-31. 2004-12-08 [PMID: 15507307] (ELISA Development, Porcine)
• Bensaude E, Turner JL, Wakeley PR, Sweetman DA, Pardieu C, Drew TW, Wileman T, Powell PP Classical swine fever virus induces proinflammatory cytokines and tissue factor expression and inhibits apoptosis and interferon synthesis during the establishment of long-term infection of porcine vascular endothelial cells. J. Gen. Virol., 2004-04-01;85(0):1029-37. 2004-04-01 [PMID: 15039545] (ELISA Development, Porcine)
• Moraes MP, Chinsangaram J, Brum MC, Grubman MJ Immediate protection of swine from foot-and-mouth disease: a combination of adenoviruses expressing interferon alpha and a foot-and-mouth disease virus subunit vaccine. Vaccine, 2003-12-12;22(2):268-79. 2003-12-12 [PMID: 14615155] (ELISA Development, Porcine)

Reviews for IFN-alpha Antibody (27105-1) (2)

Average Rating: 4.5

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Pig.

reviewed by: Verified Customer IHC-Fr Pig 01/24/2020

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: Lymph node,Placental tissue,spleen,Thymus tissue
• Species: Pig
• Lot: 6656

Comments

• Comments: Fixation: -20°C in 100% Methanol for 15 min
  Primary Ab: 1/100 (+4°C, overnight)
  Secondary: undiluted, #4001 Agilent (30 min, RT)
  Developing: AEC Substrate Kit (ab64252 Abcam) incubate for 7 mins at room temperature.
  Flood with Hematoxylin for 20 secs.

reviewed by: Verified Customer WB Pig 01/24/2020

Summary

• Application: Western Blot
• Sample Tested: Bone marrow cells,Spleen tissue
• Species: Pig
• Lot: 6656

Comments

• Comments: Sample: 10% porcine spleen lysate in RIPA supplemented with Halt™ Protease Inhibitor Cocktail.
  Gel: oxidized and denatured protein at loading. No boiling. No freezing-thawing. No pre-diluting of samples. 10% tris/glycine gel.
  Staining: blocking (5% milk, 1h), primary Ab (R&amp;D #27105-1, 1/150, overnight), secondary Ab (LI-COR #926-32210, 1/10'000, 1h).