IFN-alpha Antibody (995514) [Unconjugated]

IFN-a was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs; left panel, positive stain) and MCF-7 human breast cancer cell line (right panel, negative stain) using Mouse Anti-Human IFN-a Monoclonal Antibody (Catalog # MAB93454) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

• Reactivity: Hu
• Applications: ICC/IF
• Clone: 995514
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

IFN-alpha Antibody (995514) [Unconjugated] Summary

• Immunogen: HEK293 human embryonic kidney cell line transfected with human IFN‑ alpha 2
  Met1-Glu188
  Accession # P01563
• Specificity: Detects human IFN‑ alpha 2a,4a,6,7,8,10,16, and 17 in direct ELISAs.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IFN-alpha Antibody (995514) [Unconjugated]

• IFNA
• IFNA2
• IFNA2b
• IFNalpha
• IFN-alpha
• IFN-alphaA
• INFA2
• interferon alpha 2
• LeIF D

Background

Interferon-alpha (IFN-alpha or IFN-alpha ), also known as leukocyte interferon, represents a group of related but distinct proteins that share over 95% amino acid sequence homology. They are members of the type I interferon family which share a common cell surface receptor composed of two subunits, a 100 kDa ligand-binding subunit (IFN-alpha R2) and a 125 kDa ligand binding and signal transduction subunit (IFN-alpha R1) that is involved both in ligand binding and signal transduction. Many IFN-alpha subtypes exist, IFN-alpha 1 to IFN alpha -21. The major subtypes identified are IFN-alpha 1, IFN-alpha 2, IFN-alpha 8, IFN-alpha 10, IFN-alpha 14 and IFN-alpha 21. MAB93454 detects human IFN-alpha 2a,4a,6,7,8,10,16, and 17 in direct ELISA.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • P01563()