Recombinant Human IFN-alpha 2 Protein

• Reactivity: Hu
• Applications: Bioactivity
• Concentration: LYOPH

Recombinant Human IFN-alpha 2 Protein Summary

• Description: A single polypeptide chain containing 165 amino acids corresponding to IFN-alpha 2 Source: Yeast

  Amino Acid Sequence: CDLPQTHSLG SRRTLMLLAQ MRRISLFSCL KDRRDFGFPQ EEFGNQFQKA ETIPVLHEMI QQIFNLFSTK DSSAAWDETL LDKFYTELYQ QLNDLEACVI QGVGVTETPL MKEDSILAVR KYFQRITLYL KEKKYSPCAW EVVRAEIMRS FSLSTNLQES LRSKE

• Details of Functionality: Fully biologically active when compared to standard. The activity is determined by the cytopathic effect inhibition assay.
• Source: Yeast
• Protein/Peptide Type: Recombinant Protein
• Gene: IFNA2
• Purity: > 97 % pure by SDS-PAGE and HPLC
• Endotoxin Note: Less than 0.1 EU/ug of IFN-alpha 2 as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 19.3 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS, pH 7.4, with 0.02 % Tween-20.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: > 97 % pure by SDS-PAGE and HPLC
• Reconstitution Instructions: Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.

Alternate Names for Recombinant Human IFN-alpha 2 Protein

• alpha-2a interferon
• Ifa2
• IFNA
• IFNA2
• IFNA2a
• IFNA2b
• IFNA2c
• IFNAA
• IFNalpha 2
• IFN-alpha 2
• IFN-alpha-2
• IFN-alphaA
• INFA2
• interferon alpha 2b
• interferon alpha A
• interferon alpha-2
• Interferon alpha-A
• interferon, alpha 2
• LeIF A
• MGC125764
• MGC125765

Background

In the human genome, the IFN-omega gene family consists of seven members located on chromosome 9. However, only one of these genes is functional giving rise to the IFN-omega protein, whereas the others are non-functional pseudogenes. The IFN-omega gene is not expressed in unstimulated cells. Viral infection results in expression of the gene giving rise to the N-glycosylated protein consisting of 172 or 174 amino acids and an apparent molecular mass of about 25kDa. A single carbohydrate group consists mainly of biantennary complex oligosaccharides with variable amounts of N-acetyl neuraminic acid. In quantitative terms, IFN-omega is a major component of human leukocyte IFN; with a contribution to its total antiviral activity estimated to be in the range of 10 -15 %.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: IFNA2