IFN-alpha 2 Antibody (996586) [Unconjugated]

• Reactivity: Mu
• Applications: ELISA
• Clone: 996586
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

IFN-alpha 2 Antibody (996586) [Unconjugated] Summary

• Immunogen: Human embryonic kidney cell, HEK293-derived mouse IFN-alpha 2/IFNA2
  Cys24-Glu190
  Accession # P01573
• Specificity: Detects mouse IFN-alpha 2/IFNA2 in direct ELISA. In direct ELISAs, no cross-reactivity with mouse IFN-alpha 1, 4, 9, 11, 12 and 15 was observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IFN-alpha 2 Antibody (996586) [Unconjugated]

• alpha-2a interferon
• Ifa2
• IFNA
• IFNA2
• IFNA2a
• IFNA2b
• IFNA2c
• IFNAA
• IFNalpha 2
• IFN-alpha 2
• IFN-alpha-2
• IFN-alphaA
• INFA2
• interferon alpha 2b
• interferon alpha A
• interferon alpha-2
• Interferon alpha-A
• interferon, alpha 2
• LeIF A
• MGC125764
• MGC125765

Background

Interferon-alpha 2 (IFN alpha -2) is one of 14 subtypes within the IFN-alpha class of Type I Interferons (1). The members of the IFN-alpha class, also known as alpha leukocyte interferons, encompass a group of distinct but closely related proteins which share approximately 80% amino acid (aa) sequence identity and have a similar globular structure composed of five alpha-helices (1, 3, 4). IFN-alpha class members signal through a common cell surface receptor complex composed of IFN-alpha R2 and IFN-alpha R1 subunits (3). As the first highly active IFN to be cloned and produced, IFN alpha -2 has become the prototypic IFN for academic and pharmaceutical research (2). The mature extracellular domain (ECD) of mouse IFN alpha -2 shares 60% and 83% aa sequence identity with human and rat, respectively. Murine IFN-alpha 2 can eliminate cardiac viral load and protect cardiomyocytes from injury in animals infected with coxsackievirus B3 (CVB3) (5). IFN alpha -2 derived mutants with reduced IFNR2 binding inhibited HIV replication and mutants with more IFNAR1 binding potentiated antiviral activity (6).

1. Pestka, S. (2007) J Biol Chem. 282:20047.
2. Paul, F. et al. (2015) Gene. 567(2):132.
3. Oritani, K. et al. (2001). Cytokine & Growth Factor Reviews, 12:337.
4. Pesch, V. et al. (2004). Journal of Virology, 78:8219.
5. Wang, Y.X. et al. (2007) Am J Physiol Heart Circ Physiol. 293:H69.
6. Schlaepfer, E. et al. (2019) Am Soc for Microbiology 4:e00637.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • P01573()