IFN-alpha 2 Antibody (996586) [Alexa Fluor® 350]

• Reactivity: Mu
• Applications: ELISA
• Clone: 996586
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Alexa Fluor 350

IFN-alpha 2 Antibody (996586) [Alexa Fluor® 350] Summary

• Immunogen: Human embryonic kidney cell, HEK293-derived mouse IFN-alpha 2/IFNA2
  Cys24-Glu190
  Accession # P01573
• Specificity: Detects mouse IFN-alpha 2/IFNA2 in direct ELISA. In direct ELISAs, no cross-reactivity with mouse IFN-alpha 1, 4, 9, 11, 12 and 15 was observed.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IFN-alpha 2 Antibody (996586) [Alexa Fluor® 350]

• alpha-2a interferon
• Ifa2
• IFNA
• IFNA2
• IFNA2a
• IFNA2b
• IFNA2c
• IFNAA
• IFNalpha 2
• IFN-alpha 2
• IFN-alpha-2
• IFN-alphaA
• INFA2
• interferon alpha 2b
• interferon alpha A
• interferon alpha-2
• Interferon alpha-A
• interferon, alpha 2
• LeIF A
• MGC125764
• MGC125765

Background

Interferon-alpha 2 (IFN alpha -2) is one of 14 subtypes within the IFN-alpha class of Type I Interferons (1). The members of the IFN-alpha class, also known as alpha leukocyte interferons, encompass a group of distinct but closely related proteins which share approximately 80% amino acid (aa) sequence identity and have a similar globular structure composed of five alpha-helices (1, 3, 4). IFN-alpha class members signal through a common cell surface receptor complex composed of IFN-alpha R2 and IFN-alpha R1 subunits (3). As the first highly active IFN to be cloned and produced, IFN alpha -2 has become the prototypic IFN for academic and pharmaceutical research (2). The mature extracellular domain (ECD) of mouse IFN alpha -2 shares 60% and 83% aa sequence identity with human and rat, respectively. Murine IFN-alpha 2 can eliminate cardiac viral load and protect cardiomyocytes from injury in animals infected with coxsackievirus B3 (CVB3) (5). IFN alpha -2 derived mutants with reduced IFNR2 binding inhibited HIV replication and mutants with more IFNAR1 binding potentiated antiviral activity (6).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.