HVEM/TNFRSF14 Antibody (HMHV-1B18) - Chimeric - Azide and BSA Free

• Reactivity: Mu
• Applications: WB, Flow, IP, B/N, Func
• Clone: HMHV-1B18
• Clonality: Monoclonal
• Host: Armenian Hamster
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

HVEM/TNFRSF14 Antibody (HMHV-1B18) - Chimeric - Azide and BSA Free Summary

• Description: Novus Biologicals Armenian Hamster HVEM/TNFRSF14 Antibody (HMHV-1B18) - Chimeric - Azide and BSA Free (NBP3-09028) is a recombinant monoclonal antibody validated for use in WB, Flow and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: This antibody was raised by immunising Armenian hamsters with mouse HVEM:Fc fusion protein.
• Isotype: IgG Kappa
• Clonality: Monoclonal
• Host: Armenian Hamster
• Gene: TNFRSF14
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Block/Neutralize
  • Flow Cytometry
  • Functional Assay
  • Immunoprecipitation
  • Western Blot
• Application Notes: This chimeric hamster (Armenian) antibody was made using the variable domain sequences of the original Hamster IgG format, for improved compatibility with existing reagents, assays and techniques.
  
  This antibody has been used in FACS to demonstrate that lymphatic endothelial cells mediate deletion only via programmed cell death-1 (PD-1) ligand 1 (Tewalt et al 2012) and in Western Blot to study the role of LIGHT in the pathogenesis of hepatitis (Anand et al 2006). This antibody has been also been used in vivo experiments to study the mechanisms by which TNFSF14 functions to promote airway remodelling in asthma (Sibilano et al 2016), to confirm that costimulatory role through HVEM is not necessary for LIGHT-mediated liver inflammation (Anand et at 2006), and to investigate the role that herpesvirus entry mediator plays in the development of experimental conjunctivitis (Ishida et al, 2012). Treatment with this antibody has been observed to diminish plasma levels of antigen-specific IgG1 and IgE antibodies in mouse asthma models (Sibilano et al 2016), to interfere with the LIGHT-HVEM interaction but not interaction between B and T lymphocyte attenuator (BTLA) and HVEM in mouse hepatitis models (Anand et at 2006), and NOT to affect the development of experimental conjunctivitis in either the induction or the effector phase (Ishida et al, 2012).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Proclin 300
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for HVEM/TNFRSF14 Antibody (HMHV-1B18) - Chimeric - Azide and BSA Free

• ATAR
• CD270 antigen
• CD270
• CD40-like protein
• Herpes virus entry mediator A
• Herpesvirus entry mediator A
• HveA
• HVEM
• HVEMTR2HVEAATAR
• LIGHTR
• TNFRSF14
• tumor necrosis factor receptor superfamily member 14
• tumor necrosis factor receptor superfamily, member 14 (herpesvirus entrymediator)
• Tumor necrosis factor receptor-like 2
• tumor necrosis factor receptor-like gene2

Background

TNFRSF14 is encoded by this gene is a member of the TNF-receptor superfamily. This receptor was identified as a cellular mediator of herpes simplex virus (HSV) entry. Binding of HSV viral envelope glycoprotein D (gD) to this receptor protein has been shown to be part of the viral entry mechanism. The cytoplasmic region of this receptor was found to bind to several TRAF family members, which may mediate the signal transduction pathways that activate the immune response.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TNFRSF14