HUWE1 Antibody

Product Discontinued

HUWE1 Antibody Summary

• Immunogen: Peptide with sequence C-DDLKSNTEYHKYQ corresponding to internal region according to NP_113584.3.
• Epitope: internal region
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: HUWE1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:128000
• Application Notes: WB: Not yet tested - Our standard WB protocol would not expect to reliably detect proteins as large as the calculated size of 482 kDa band according to NP_113584.3. Therefore we cannot recommend an optimal concentration and the antibody is an aspiring p

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Notes

Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.

Alternate Names for HUWE1 Antibody

• arf-bp1
• EC 6.3.2
• EC 6.3.2.6
• HECT, UBA and WWE domain containing 1
• HECTH9
• HSPC272
• KIAA0312MULE
• Mcl-1 ubiquitin ligase E3
• Mule
• UREB1UBA and WWE domain-containing protein 1
• URE-binding protein 1

Background

ARFBP1 is a novel E3 ubiquitin ligase. It interacts with p19ARF and its ligase activity is inhibited by this interaction.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HUWE1 Antibody (NBP1-45233)(1)

Publication using NBP1-45233

• Stankiewicz AR, Livingstone AM, Mohseni N, Mosser DD. Regulation of heat-induced apoptosis by Mcl-1 degradation and its inhibition by Hsp70 Cell Death Differ 2009-01-17 [PMID: 19148187]
  
  Details:
  Citation using the Non-Recombinant Monoclonal and Biotin format of this antibody.

Bioinformatics

• Gene Symbol: HUWE1
• Entrez:
  • 10075(Human)
  • 59026(Mouse)
• Uniprot:
  • NP_113584.3(Human)