Human IL-28B/IFN-lambda 3 Quantikine ELISA Kit

• Reactivity: Hu
• Applications: ELISA
• Conjugate: HRP

Human IL-28B/IFN-lambda 3 Quantikine ELISA Kit Summary

• Background: The Quantikine Human IL-28B/IFN-lambda 3 Immunoassay is a 4.5 hour solid phase ELISA designed to measure IL-28B levels in cell culture supernates, cell lysates, serum, EDTA plasma, and urine. It contains CHO cell-expressed recombinant human IL-28B and antibodies raised against the recombinant protein. Results obtained for naturally occurring human IL-28B showed linear curves that were parall...el to the standard curves obtained using the Quantikine Human IL-28B Immunoassay standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-28B.
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• Specificity: Natural and recombinant human IL-28B.
• Source: N/A
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: IFNL3

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human IL-28B/IFN-lambda 3 Quantikine ELISA Kit

• interleukin-28B
• IFN-lambda 3
• IL28B
• IL-28B
• IL28C
• interferon, lambda 3

Background

Human/mouse IL-28A (IFN-lambda 2), IL-28B (IFN-lambda 3), and human IL-29 (IFN-lambda 1) are class II cytokine receptor ligands that are distantly related to members of the IL-10 family (11 - 13% amino acid (aa) sequence identity) and type I IFN family (15 - 19% aa sequence identity). These cytokines exert bioactivities that overlap those of type I IFNs, including antiviral activity and up-regulation of MHC class I antigen expression. The proteins signal through the same heterodimeric receptor complex that is composed of the IL-10 receptor beta (IL-10 R beta) and a novel IL-28 receptor alpha (IL-28 R alpha, also known as IFN-lambda R1). Mouse IL-28 shares 61%, 62%, and 52% aa identity with human IL-28A, IL-28B, and IL-29, respectively.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for IL-28B/IFN-lambda 3 (D28B00)(5)

Publications using D28B00

• Maeda, R;Seki, N;Uwamino, Y;Wakui, M;Nakagama, Y;Kido, Y;Sasai, M;Taira, S;Toriu, N;Yamamoto, M;Matsuura, Y;Uchiyama, J;Yamaguchi, G;Hirakawa, M;Kim, YG;Mishima, M;Yanagita, M;Suematsu, M;Sugiura, Y; Amino acid catabolite markers for early prognostication of pneumonia in patients with COVID-19 Nature communications 2023-12-20 [PMID: 38123556] (Human)
• M Hagihara, M Yamashita, T Ariyoshi, S Eguchi, A Minemura, D Miura, S Higashi, K Oka, T Nonogaki, T Mori, K Iwasaki, J Hirai, Y Shibata, T Umemura, H Kato, N Asai, Y Yamagishi, A Ota, M Takahashi, H Mikamo Clostridium butyricum-induced ?-3 fatty acid 18-HEPE elicits anti-influenza virus pneumonia effects through interferon-lambda upregulation Cell Reports, 2022-12-13;41(11):111755. 2022-12-13 [PMID: 36516771] (Human)
• U Rand, SY Kupke, H Shkarlet, MD Hein, T Hirsch, P Marichal-G, L Cicin-Sain, U Reichl, D Bruder Antiviral Activity of Influenza A Virus Defective Interfering Particles against SARS-CoV-2 Replication In Vitro through Stimulation of Innate Immunity Cells, 2021-07-11;10(7):. 2021-07-11 [PMID: 34359926] (Human)
• J Xing, X Zhou, M Fang, E Zhang, LJ Minze, Z Zhang DHX15 is required to control RNA virus-induced intestinal inflammation Cell Reports, 2021-06-22;35(12):109205. 2021-06-22 [PMID: 34161762] (Human)
• PJ Metz, KA Ching, T Xie, P Delgado Cu, S Niessen, JH Tatlock, K Jensen-Per, BW Murray Symmetric Arginine Dimethylation Is Selectively Required for mRNA Splicing and the Initiation of Type I and Type III Interferon Signaling Cell Rep, 2020-02-11;30(6):1935-1950.e8. 2020-02-11 [PMID: 32049022] (Human)

Bioinformatics

• Gene Symbol: IFNL3