Human IL-28B/IFN-lambda 3 Quantikine ELISA Kit

• Reactivity: Hu
• Applications: ELISA
• Conjugate: HRP

Human IL-28B/IFN-lambda 3 Quantikine ELISA Kit Summary

• Background: The Quantikine Human IL-28B/IFN-lambda 3 Immunoassay is a 4.5 hour solid phase ELISA designed to measure IL-28B levels in cell culture supernates, cell lysates, serum, EDTA plasma, and urine. It contains CHO cell-expressed recombinant human IL-28B and antibodies raised against the recombinant protein. Results obtained for naturally occurring human IL-28B showed linear curves that were parall...el to the standard curves obtained using the Quantikine Human IL-28B Immunoassay standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-28B.
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• Specificity: Natural and recombinant human IL-28B.
• Source: N/A
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: IFNL3

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human IL-28B/IFN-lambda 3 Quantikine ELISA Kit

• interleukin-28B
• IFN-lambda 3
• IL28B
• IL-28B
• IL28C
• interferon, lambda 3

Background

Human/mouse IL-28A (IFN-lambda 2), IL-28B (IFN-lambda 3), and human IL-29 (IFN-lambda 1) are class II cytokine receptor ligands that are distantly related to members of the IL-10 family (11 - 13% amino acid (aa) sequence identity) and type I IFN family (15 - 19% aa sequence identity). These cytokines exert bioactivities that overlap those of type I IFNs, including antiviral activity and up-regulation of MHC class I antigen expression. The proteins signal through the same heterodimeric receptor complex that is composed of the IL-10 receptor beta (IL-10 R beta) and a novel IL-28 receptor alpha (IL-28 R alpha, also known as IFN-lambda R1). Mouse IL-28 shares 61%, 62%, and 52% aa identity with human IL-28A, IL-28B, and IL-29, respectively.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for IL-28B/IFN-lambda 3 (D28B00)(5)

Publications using D28B00

• Maeda, R;Seki, N;Uwamino, Y;Wakui, M;Nakagama, Y;Kido, Y;Sasai, M;Taira, S;Toriu, N;Yamamoto, M;Matsuura, Y;Uchiyama, J;Yamaguchi, G;Hirakawa, M;Kim, YG;Mishima, M;Yanagita, M;Suematsu, M;Sugiura, Y; Amino acid catabolite markers for early prognostication of pneumonia in patients with COVID-19 Nature communications 2023-12-20 [PMID: 38123556] (Human)
• M Hagihara, M Yamashita, T Ariyoshi, S Eguchi, A Minemura, D Miura, S Higashi, K Oka, T Nonogaki, T Mori, K Iwasaki, J Hirai, Y Shibata, T Umemura, H Kato, N Asai, Y Yamagishi, A Ota, M Takahashi, H Mikamo Clostridium butyricum-induced ?-3 fatty acid 18-HEPE elicits anti-influenza virus pneumonia effects through interferon-lambda upregulation Cell Reports, 2022-12-13;41(11):111755. 2022-12-13 [PMID: 36516771] (Human)
• U Rand, SY Kupke, H Shkarlet, MD Hein, T Hirsch, P Marichal-G, L Cicin-Sain, U Reichl, D Bruder Antiviral Activity of Influenza A Virus Defective Interfering Particles against SARS-CoV-2 Replication In Vitro through Stimulation of Innate Immunity Cells, 2021-07-11;10(7):. 2021-07-11 [PMID: 34359926] (Human)
• J Xing, X Zhou, M Fang, E Zhang, LJ Minze, Z Zhang DHX15 is required to control RNA virus-induced intestinal inflammation Cell Reports, 2021-06-22;35(12):109205. 2021-06-22 [PMID: 34161762] (Human)
• PJ Metz, KA Ching, T Xie, P Delgado Cu, S Niessen, JH Tatlock, K Jensen-Per, BW Murray Symmetric Arginine Dimethylation Is Selectively Required for mRNA Splicing and the Initiation of Type I and Type III Interferon Signaling Cell Rep, 2020-02-11;30(6):1935-1950.e8. 2020-02-11 [PMID: 32049022] (Human)

FAQs for IL-28B/IFN-lambda 3 (D28B00). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: IFNL3