Human IgG4 Antibody (2350A) [Unconjugated]

Images

 
Recombinant Human IgG4 protein was serially diluted 2-fold and captured by Rabbit Anti-Human IgG4 Monoclonal Antibody (Catalog # MAB98951) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rabbit ...read more
Western blot shows lysates of purified human IgG1 (negative control), purified human IgG2 (negative control), purified human IgG3 (negative control), and human purified human IgG4. PVDF membrane was probed with ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA
Clone
2350A
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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Catalog# & Formulation Size Price

Human IgG4 Antibody (2350A) [Unconjugated] Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
Purified IgG4 from normal human plasma
Specificity
Detects human IgG4 in Western blots and ELISA. In Western blots, no cross-reactivity with human IgG1, human IgG2, and human IgG3.
Source
N/A
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Purity Statement
Protein A or G purified from cell culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human IgG4 Antibody (2350A) [Unconjugated]

  • Ig gamma-4 chain C region
  • IgG4
  • immunoglobulin heavy constant gamma 4 (G4m marker)
  • MGC117419

Background

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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