Human IFN-alpha Multi-Subtype ELISA Kit

• Reactivity: Hu
• Applications: ELISA
• Conjugate: Biotin

Human IFN-alpha Multi-Subtype ELISA Kit Summary

• Specificity: Human IFN-alpha
• Source: N/A
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: IFNA2

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human IFN-alpha Multi-Subtype ELISA Kit

• IFNA
• IFNA2
• IFNA2b
• IFNalpha
• IFN-alpha
• IFN-alphaA
• INFA2
• interferon alpha 2
• LeIF D

Background

Interferon-alpha (IFN-alpha), also known as leukocyte interferon, represents a group of related but distinct proteins that share over 95% amino acid sequence homology. They are members of the type I interferon family which share a common cell surface receptor composed of two subunits, a 100 kDa ligand-binding subunit (IFN-alpha R2) and a 125 kDa ligand binding and signal transduction subunit (IFN-alpha R1) that is involved both in ligand binding and signal transduction. IFN-alpha has both anti-viral and immunomodulatory activities on target cells.

Publications for IFN-alpha (41105-1)(4)

Publications using 41105-1

• Stone , Amy E L, Giugliano , Silvia, Schnell , Gretja, Cheng , Linling, Leahy , Katelyn, Golden-Mason , Lucy, Gale , Michael, Rosen , Hugo R Hepatitis C virus pathogen associated molecular pattern (PAMP) triggers production of lambda-interferons by human plasmacytoid dendritic cells. PLoS Pathog, 2013-04-18;9(4):e1003316. 2013-04-18 [PMID: 23637605] (Human)
• Chen , Qian, Swaminathan , Sanjay, Yang , De, Dai , Lue, Sui , Hongyan, Yang , Jun, Hornung , Ronald L, Wang , Yanmei, Huang , Da Wei, Hu , Xiaojun, Lempicki , Richard, Imamichi , Tomozumi Interleukin-27 is a potent inhibitor of cis HIV-1 replication in monocyte-derived dendritic cells via a type I interferon-independent pathway. PLoS ONE, 2013-03-20;8(3):e59194. 2013-03-20 [PMID: 23527130] (Human)
• Zepp JA, Nold-Petry CA, Dinarello CA, Nold MF Protection from RNA and DNA viruses by IL-32. J. Immunol., 2011-02-23;186(7):4110-8. 2011-02-23 [PMID: 21346229] (Human)
• Nold MF, Nold-Petry CA, Pott GB, Zepp JA, Saavedra MT, Kim SH, Dinarello CA Endogenous IL-32 controls cytokine and HIV-1 production. J. Immunol., 2008-07-01;181(1):557-65. 2008-07-01 [PMID: 18566422] (Human)

FAQs for IFN-alpha (41105-1). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: IFNA2