Human Granzyme A DuoSet ELISA, 5 Plate

• Reactivity: Hu
• Applications: ELISA
• Conjugate: Biotin

Human Granzyme A DuoSet ELISA, 5 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human Granzyme A DuoSet ELISA, 5 Plate

• CTL Tryptase
• CTLA3
• CTLA3Granzyme A (Cytotoxic T-lymphocyte-associated serine esterase-3; Hanukah factorserine protease)
• Cytotoxic T-lymphocyte proteinase 1
• EC 3.4.21
• Fragmentin-1
• granzyme A (granzyme 1, cytotoxic T-lymphocyte-associated serine esterase 3)
• Granzyme A
• Granzyme-1
• GZMA
• H factor
• Hanukkah factor
• HF
• HFSP
• HFSPEC 3.4.21.78

Background

Granzyme family serine proteases are stored in the granules of cytotoxic T lymphocytes and natural killer cells. Granzymes contain one S1 protease domain and are synthesized as preproproteins. Granzymes are released toward pathogen infected or transformed target cells during cellular immune reactions. Target cell entry by granzymes is through perforin channels. Granzymes trigger apoptosis by caspase-dependent and -independent mechanisms. There are five granzymes (A, B, G, H and K) in human and many more in mouse and rat.

Publications for Granzyme A (DY2905-05)(4)

Publications using DY2905-05

• Wu, WC;Shiu, C;Tong, TK;Leung, SO;Hui, CW; Suppression of NK Cell Activation by JAK3 Inhibition: Implication in the Treatment of Autoimmune Diseases Journal of immunology research 2023-12-08 [PMID: 38106519] (Human)
• Tsilioni, I;Theoharides, TC; Recombinant SARS-CoV-2 Spike Protein Stimulates Secretion of Chymase, Tryptase, and IL-1? from Human Mast Cells, Augmented by IL-33 International journal of molecular sciences 2023-05-30 [PMID: 37298438] (Human)
• J Friebel, M Witkowski, M Wegner, L Blöbaum, S Lammel, PA Schencke, K Jakobs, M Puccini, D Rei beta ner, D Steffens, V Moos, HP Schutheiss, U Landmesser, U Rauch Cytotoxic CD8+ T Cells Are Involved in the Thrombo-Inflammatory Response during First-Diagnosed Atrial Fibrillation Cells, 2022-12-29;12(1):. 2022-12-29 [PMID: 36611934] (Human)
• V Yekehfalla, S Pahlavanne, A Sayadmanes, Z Momtahan, B Ma, M Basiri Generation and Functional Characterization of PLAP CAR-T Cells against Cervical Cancer Cells Biomolecules, 2022-09-14;12(9):. 2022-09-14 [PMID: 36139135] (Human)

FAQs for Granzyme A (DY2905-05). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.