Human FAP DuoSet ELISA, 15 Plate

• Reactivity: Hu
• Applications: ELISA
• Conjugate: Biotin

Human FAP DuoSet ELISA, 15 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: FAP

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human FAP DuoSet ELISA, 15 Plate

• 170 kDa melanoma membrane-bound gelatinase
• DKFZp686G13158
• DPPIV
• EC 3.4.21.-
• FAP
• FAPA
• Fibroblast Activation Protein alpha
• fibroblast activation protein, alpha
• Integral membrane serine protease
• Seprase
• vibronectin

Background

FAP (fibroblast activation protein-alpha; also known as seprase) is a type II transmembrane serine protease that degrades gelatin, Type I collagen, and N-terminal prolines. FAP is induced on reactive stromal fibroblasts in epithelial cancers, sarcomas, and granulation tissue, and may play roles in tumor invasion, tissue remodeling, and wound repair.

Publications for Fibroblast Activation Protein alpha/FAP (DY3715)(11)

Publications using DY3715

• Hemmingsen, JK;Enemark, MH;Pedersen, AKN;Sørensen, EF;Lauridsen, KL;Løhde, JB;d'Amore, F;Hamilton-Dutoit, SJ;Bjerre, M;Ludvigsen, M; Reduced Activity of Soluble Fibroblast Activation Protein (sFAP) Represents a Biomarker of Aggressive Disease in Lymphoid Malignancies International journal of molecular sciences 2025-11-21 [PMID: 41373408] (Human)
• Lee, HJ;Jo, Y;Wei, S;Yu, EH;Lee, S;Ryu, D;Joo, JK; Fibroblast Activation Protein (FAP) as a Serum Biomarker for Fibrotic Ovarian Aging: A Clinical Validation Study Based on Translational Transcriptomic Targets International journal of molecular sciences 2025-08-13 [PMID: 40869127] (Human)
• Z Yuan, H Hu, Y Zhu, W Zhang, Q Fang, T Qiao, T Ma, M Wang, R Huang, Q Tang, F Gao, C Zou, X Gao, G Wang, X Wang Colorectal cancer cell intrinsic fibroblast activation protein alpha binds to Enolase1 and activates NF-&kappaB pathway to promote metastasis Cell Death & Disease, 2021-05-25;12(6):543. 2021-05-25 [PMID: 34035230] (Human)
• B Baráth, R Bogáti, T Miklós, J Kállai, ZA Mezei, Z Bereczky, L Muszbek, É Katona Effect of &alpha2-plasmin inhibitor heterogeneity on the risk of venous thromboembolism Thrombosis Research, 2021-05-11;203(0):110-116. 2021-05-11 [PMID: 33992873] (Human)
• Z Bagoly, B Baráth, R Orbán-Kálm, I Szegedi, R Bogáti, F Sarkady, L Csiba, É Katona Incorporation of &alpha2-Plasmin Inhibitor into Fibrin Clots and Its Association with the Clinical Outcome of Acute Ischemic Stroke Patients Biomolecules, 2021-02-25;11(3):. 2021-02-25 [PMID: 33669007] (Human)
• E Krepela, Z Vanickova, P Hrabal, M Zubal, B Chmielova, E Balaziova, P Vymola, I Matrasova, P Busek, A Sedo Regulation of Fibroblast Activation Protein by Transforming Growth Factor Beta-1 in Glioblastoma Microenvironment International Journal of Molecular Sciences, 2021-01-21;22(3):. 2021-01-21 [PMID: 33494271] (Human)
• JD Solano-Itu, P Errarte, MC Etxezarrag, E Echevarria, J Angulo, JI López, G Larrinaga Altered Tissue and Plasma Levels of Fibroblast Activation Protein-&alpha (FAP) in Renal Tumours Cancers (Basel), 2020-11-16;12(11):. 2020-11-16 [PMID: 33207686] (Human)
• J Liu, Z Zuo, M Zou, T Finkel, S Liu Identification of the transcription factor Miz1 as an essential regulator of diphthamide biosynthesis using a CRISPR-mediated genome-wide screen PLoS Genet, 2020-10-15;16(10):e1009068. 2020-10-15 [PMID: 33057331] (Human)
• TW Bainbridge, DR Dunshee, NM Kljavin, NJ Skelton, J Sonoda, JA Ernst Selective Homogeneous Assay for Circulating Endopeptidase Fibroblast Activation Protein (FAP) Sci Rep, 2017-10-02;7(1):12524. 2017-10-02 [PMID: 28970566] (Human)
• S Uitte de W, FM Keane, DG Bowen, JJMC Malfliet, HE Zhang, B Maneck, GW McCaughan, FWG Leebeek, DC Rijken, MD Gorrell Circulating fibroblast activation protein activity and antigen levels correlate strongly when measured in liver disease and coronary heart disease PLoS ONE, 2017-06-05;12(6):e0178987. 2017-06-05 [PMID: 28582421] (Human)
• Matrasova I, Busek P, Balaziova E, Sedo A Heterogeneity of molecular forms of dipeptidyl peptidase-IV and fibroblast activation protein in human glioblastomas. Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub, 2017-04-26;161(3):252-260. 2017-04-26 [PMID: 28452380] (Human)

FAQs for Fibroblast Activation Protein alpha/FAP (DY3715). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: FAP