Human ENPP-2/Autotaxin DuoSet ELISA, 5 Plate

• Reactivity: Hu
• Applications: ELISA
• Conjugate: Biotin

Human ENPP-2/Autotaxin DuoSet ELISA, 5 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: ENPP2

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human ENPP-2/Autotaxin DuoSet ELISA, 5 Plate

• ATX
• ATXFLJ26803
• ATX-X
• Autotaxin
• autotaxin-t
• EC 3.1.4.39
• ectonucleotide pyrophosphatase/phosphodiesterase 2
• ectonucleotide pyrophosphatase/phosphodiesterase family member 2
• E-NPP 2
• ENPP2
• ENPP-2
• Extracellular lysophospholipase D
• Lysophosphatidic Acid
• LysoPLD
• NPP2
• PD-IALPHA
• PDNP2
• PDNP2NPP2
• phosphodiesterase I/nucleotide pyrophosphatase 2
• plasma lysophospholipase D

Background

ENPP-2, also known as Autotaxin, belongs to the ectonucleotide pyrophosphatase/phosphodiesterase (NPP) family. Some NPPs hydrolyze phosphates from nucleotides and their derivatives. ENPP-2 shares 40 - 50% identity to ENPP1 & 3, all of which contain a N-terminal intracellular domain, a single transmembrane domain and a large extracellular domain that includes a catalytic domain, two somatomedin-B-like domains, and a C-terminal nuclease-like domain. Unlike ENPP-1 and ENPP-3, ENPP-2 has weak activity against nucleotides, but exhibits a lysophospholipase D activity which allows the formation of lysophosphatidic acid (LPA) and choline from lysophosphatidylcholine. The hydrolysis of nucleotides and lysophospholipids by ENPP-2 is mediated by a single catalytic site. Evidence shows LPA and sphingosine 1-phosphate to be specific inhibitors of ENPP-2. ENPP-2 was originally found to stimulate tumor cell motility and has since been found to enhance tumor invasion and metastasis and to be up-regulated in several types of carcinomas including breast and lung.

Publications for ENPP-2/Autotaxin (DY5255-05)(3)

Publications using DY5255-05

• I Nikitopoul, A Katsifa, P Kanellopou, E Jahaj, AG Vassiliou, Z Mastora, I Dimopoulou, SE Orfanos, V Aidinis, A Kotanidou Autotaxin Has a Negative Role in Systemic Inflammation International Journal of Molecular Sciences, 2022-07-18;23(14):. 2022-07-18 [PMID: 35887265] (Human)
• R Schmid, K Wolf, JW Robering, S Strau beta , PL Strissel, R Strick, M Rübner, PA Fasching, RE Horch, AE Kremer, AM Boos, A Weigand ADSCs and adipocytes are the main producers in the autotaxin-lysophosphatidic acid axis of breast cancer and healthy mammary tissue in vitro BMC Cancer, 2018-12-19;18(1):1273. 2018-12-19 [PMID: 30567518] (Human)
• Serum Autotaxin is a Marker of the Severity of Liver Injury and Overall Survival in Patients with Cholestatic Liver Diseases Sci Rep, 2016-08-10;6(0):30847. 2016-08-10 [PMID: 27506882] (Human)

FAQs for ENPP-2/Autotaxin (DY5255-05). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: ENPP2