Recombinant Human HSP40/DNAJB1 Protein

Product Discontinued

Recombinant Human HSP40/DNAJB1 Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to amino acids 1 to 340 of Human HSP40/DNAJB1
  
  Source: E.coli
  
  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGKDYYQTLG LARGASDEEI KRAYRRQALR YHPDKNKEPG AEEKFKEIAE AYDVLSDPRK REIFDRYGEE GLKGSGPSGG SGGGANGTSF SYTFHGDPHA MFAEFFGGRN PFDTFFGQRN GEEGMDIDDP FSGFPMGMGG FTNVNFGRSR SAQEPARKKQ DPPVTHDLRV SLEEIYSGCT KKMKISHKRL NPDGKSIRNE DKILTIEVKK GWKEGTKITF PKEGDQTSNN IPADIVFVLK DKPHNIFKRD GSDVIYPARI SLREALCGCT VNVPTLDGRT IPVVFKDVIR PGMRRKVPGE GLPLPKTPEK RGDLIIEFEV IFPERIPQTS RTVLEQVLPI
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: DNAJB1
• Purity: >95%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Application Notes: Use in In vitro reported in scientific literature (PMID 26443817)
• Theoretical MW: 40.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris buffer (pH 8.0), 2 mM DTT, 0.2 mM PMSF, 10% glycerol
• Preservative: No Preservative
• Concentration: 1.0 mg/ml
• Purity: >95%, by SDS-PAGE

Alternate Names for Recombinant Human HSP40/DNAJB1 Protein

• DnaJ (Hsp40) homolog, subfamily B, member 1
• DnaJ (Hsp40) homolog, subfmaily B, member 1
• dnaJ homolog subfamily B member 1
• DnaJ protein homolog 1
• DNAJ1
• DNAJB1
• Hdj1
• hDj-1
• Heat shock 40 kDa protein 1
• heat shock 40kD protein 1
• Heat shock protein 40
• HSP40
• HSPF1
• HSPF1Hdj1
• Human DnaJ protein 1
• radial spoke 16 homolog B
• RSPH16B
• Sis1

Background

Human Hsp40/DNAJB1 (heat shock protein with molecular size of 40kDa) is one of the mammalian homologues of bacterial DnaJ heat shock protein and regulates cellular processes by aiding in the folding, transport and assembly. HSP40 protein contains the J-domain which controls interaction with the ATPase domain of DnaK. Recombinant human Hsp40/DNAJB1, his-tagged, is overexpressed in E.coli and purified by using the conventional column chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: DNAJB1