HSP40/DNAJB1 Antibody (2A7.H6)

Images

 
Western Blot: HSP40/DNAJB1 Antibody (2A7.H6) [NBP1-22416] - analysis of Yeast recombinant cell lysate showing detection of Hsp40 protein using Mouse Anti-Hsp40 Monoclonal Antibody, Clone 2A7.H6. Load: 15 ug protein. ...read more

Product Details

Summary
Reactivity YeSpecies Glossary
Applications WB, ELISA, IP
Clone
2A7.H6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

HSP40/DNAJB1 Antibody (2A7.H6) Summary

Immunogen
Full length protein HSP40 (YDJ1).
Localization
Cytoplasm, Nucleus
Specificity
Detects approx 40kDa. Yeast specific.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
DNAJB1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000
  • ELISA 1:100-1:2000
  • Immunoprecipitation 1:10-1:500
Application Notes
0.5 ug/ml of HSP40, YDJ1 Antibody was sufficient for detection of 50 ng YDJ1 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.
Reviewed Applications
Read 1 Review rated 4
using
NBP1-22416 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
50% Glycerol
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for HSP40/DNAJB1 Antibody (2A7.H6)

  • DnaJ (Hsp40) homolog, subfamily B, member 1
  • DnaJ (Hsp40) homolog, subfmaily B, member 1
  • dnaJ homolog subfamily B member 1
  • DnaJ protein homolog 1
  • DNAJ1
  • DNAJB1
  • Hdj1
  • hDj-1
  • Heat shock 40 kDa protein 1
  • heat shock 40kD protein 1
  • Heat shock protein 40
  • HSP40
  • HSPF1
  • HSPF1Hdj1
  • Human DnaJ protein 1
  • radial spoke 16 homolog B
  • RSPH16B
  • Sis1

Background

Human Hsp40/DnaJ proteins comprise a large protein family, members of which feature the J domain (named after the bacterial DnaJ protein) (1). The J-domain spans the first 75 N-terminal amino acids and is separated from the C-terminal by a glycine/phenylalanine-rich domain (2). There are two main types of Hsp40; type 1 DNAJ proteins including HDJ2 and yeast YdjI; type II includes yeast Sis1 and human Hdj1. Whereas type I possesses a zinc finger domain which helps in the function of protein folding. (3, 4), type II does not. Members of the Hsp40/DnaJ family play diverse roles in many cellular processes, such as folding, translocation, degradation and assembly of multi-protein complexes. Hsp40 stimulates the ATPase activity of Hsp70 which in turn causes conformational changes of the unfolded proteins (5, 6). The Hsp40-Hsp70-unfolded protein complex further binds to co-chaperones Hip, Hop and HSP90 which leads to protein folding, or components of protein degradation machinery CHIP and BAG-1 (7).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-81696
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-02996
Species: Hu, Rt, Ca, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-P
H00010049-M01
Species: Hu
Applications: WB, ELISA, ICC/IF
NBP1-97868
Species: Hu, Mu, Rt, Po, Bv, Ca, Ch, GP, Ha, Mk, Rb, Sh
Applications: WB, EM, EIA, Flow, ICC/IF, IHC, IHC-P, IP
NB120-2928
Species: Hu, Mu, Rt, Pm, Rb, Sh
Applications: WB, ICC/IF, IHC, IHC-P, IP
NB600-952
Species: Hu, Mu, Rt, Po, Ba, Ha, Pm, Rb
Applications: WB, Flow, IHC, IHC-P, IP, IF
NBP1-02459
Species: Hu, Mu, Rt, Ch, Dr, Pm
Applications: WB, ELISA, IHC, IHC-P
NBP1-77397
Species: Hu, Mu, Rt, Ha, Pm
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-P
H00010963-M01
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, IP
NBP2-32972
Species: Hu, Mu, Rt, Ch, Pm, Sh
Applications: WB, Flow, ICC/IF, IHC-Fr, IHC-P, IP, PAGE
NB120-2813
Species: Hu, Mu, Ch, Rb
Applications: WB, ICC/IF, IP
NB100-56703
Species: Hu, Mu
Applications: WB, IHC, IHC-Fr
NB300-576
Species: Hu, Mu, Rt, Ch, GP, Pm, Rb, Xp
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
NBP1-33548
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P, IP
NBP1-97503
Species: Hu, Mu, Rt, Po, Bv, Ca, Ch, GP, Ha, Md, Pm, Rb, Sh, Xp
Applications: WB, IHC, IHC-P, IP
NBP1-06274
Species: Hu, Mu, Rt, Ch, Dr, Sh
Applications: WB, Simple Western, Flow, ICC/IF
AF7250
Species: Hu
Applications: IHC
NB110-57586
Species: Hu, Mu, Rt, Bv, Ch, Pm, Rb
Applications: WB, IHC, IHC-P
AF4029
Species: Hu, Mu, Rt
Applications: WB, IHC
NB100-56161
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P, IP

Publications for HSP40/DNAJB1 Antibody (NBP1-22416) (0)

There are no publications for HSP40/DNAJB1 Antibody (NBP1-22416).
By submitting your publication information earn gift cards and discounts for future purchases.

Review for HSP40/DNAJB1 Antibody (NBP1-22416) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Yeast
.
We have 1 review tested in 1 application: WB.

Reviews using NBP1-22416:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Yeast
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot HSP40/DNAJB1 NBP1-22416
Enlarge
reviewed by:
Anonymous
WB Yeast
 08/18/2017
View

Summary

ApplicationWestern Blot
Sample Testedwhole cell lysate
SpeciesYeast
Lot0811

Comments

Comments4-12% Bis-Tris gel, 30ug of protein. Incubated o/n at 4C 1:1000 in milk.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for HSP40/DNAJB1 Antibody (NBP1-22416) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional HSP40/DNAJB1 Products

Bioinformatics Tool for HSP40/DNAJB1 Antibody (NBP1-22416)

Discover related pathways, diseases and genes to HSP40/DNAJB1 Antibody (NBP1-22416). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for HSP40/DNAJB1 Antibody (NBP1-22416)

Discover more about diseases related to HSP40/DNAJB1 Antibody (NBP1-22416).
 

Pathways for HSP40/DNAJB1 Antibody (NBP1-22416)

View related products by pathway.

PTMs for HSP40/DNAJB1 Antibody (NBP1-22416)

Learn more about PTMs related to HSP40/DNAJB1 Antibody (NBP1-22416).

Blogs on HSP40/DNAJB1

There are no specific blogs for HSP40/DNAJB1, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

Anonymous
08/18/2017
Application: WB
Species: Yeast

Bioinformatics

Gene Symbol DNAJB1
Entrez
Uniprot