HSP40/DNAJB1 Antibody (2A7.H6) - BSA Free

Western Blot: HSP40/DNAJB1 Antibody (2A7.H6) [NBP1-22416] - Western Blot analysis of Yeast recombinant cell lysate showing detection of Hsp40 protein using Mouse Anti-Hsp40 Monoclonal Antibody, Clone 2A7.H6 (NBP1-22416). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp40 Monoclonal Antibody (NBP1-22416) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.

Western Blot: HSP40/DNAJB1 Antibody (2A7.H6) [NBP1-22416] - 30ug of protein. Antibody dilutuion of 1:1000. This image was submitted via customer Review.

• Reactivity: Ye
• Applications: WB, ELISA, IP
• Clone: 2A7.H6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

HSP40/DNAJB1 Antibody (2A7.H6) - BSA Free Summary

• Description: Novus Biologicals Mouse HSP40/DNAJB1 Antibody (2A7.H6) - BSA Free (NBP1-22416) is a monoclonal antibody validated for use in WB, ELISA and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length protein HSP40 (YDJ1)
• Localization: Cytoplasm , Nucleus
• Specificity: Detects approx 40kDa. Yeast specific.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: DNAJB1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunoprecipitation
  • Western Blot 1:2000
• Application Notes: 0.5 ug/ml of HSP40, YDJ1 Antibody was sufficient for detection of 50 ng YDJ1 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50% Glycerol
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for HSP40/DNAJB1 Antibody (2A7.H6) - BSA Free

• DnaJ (Hsp40) homolog, subfamily B, member 1
• DnaJ (Hsp40) homolog, subfmaily B, member 1
• dnaJ homolog subfamily B member 1
• DnaJ protein homolog 1
• DNAJ1
• DNAJB1
• Hdj1
• hDj-1
• Heat shock 40 kDa protein 1
• heat shock 40kD protein 1
• Heat shock protein 40
• HSP40
• HSPF1
• HSPF1Hdj1
• Human DnaJ protein 1
• radial spoke 16 homolog B
• RSPH16B
• Sis1

Background

Human Hsp40/DnaJ proteins comprise a large protein family, members of which feature the J domain (named after the bacterial DnaJ protein) (1). The J-domain spans the first 75 N-terminal amino acids and is separated from the C-terminal by a glycine/phenylalanine-rich domain (2). There are two main types of Hsp40; type 1 DNAJ proteins including HDJ2 and yeast YdjI; type II includes yeast Sis1 and human Hdj1. Whereas type I possesses a zinc finger domain which helps in the function of protein folding. (3, 4), type II does not. Members of the Hsp40/DnaJ family play diverse roles in many cellular processes, such as folding, translocation, degradation and assembly of multi-protein complexes. Hsp40 stimulates the ATPase activity of Hsp70 which in turn causes conformational changes of the unfolded proteins (5, 6). The Hsp40-Hsp70-unfolded protein complex further binds to co-chaperones Hip, Hop and HSP90 which leads to protein folding, or components of protein degradation machinery CHIP and BAG-1 (7).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for HSP40/DNAJB1 Antibody (NBP1-22416) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Yeast.

reviewed by: Verified Customer WB Yeast 08/18/2017

Summary

• Application: Western Blot
• Sample Tested: whole cell lysate
• Species: Yeast
• Lot: 0811

Comments

• Comments: 4-12% Bis-Tris gel, 30ug of protein. Incubated o/n at 4C 1:1000 in milk.

Bioinformatics

• Gene Symbol: DNAJB1
• Entrez:
  • 3337(Human)
• Uniprot:
  • P25491(Yeast)