Mouse HSF2 ELISA Kit (Colorimetric)

ELISA: Mouse HSF2 ELISA Kit (Colorimetric) [NBP2-76592] - Samples were spiked with high concentrations of Mouse HSF2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Mouse HSF2 ELISA Kit (Colorimetric) [NBP2-76592] - Standard Curve Reference

• Reactivity: Mu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 7.813 - 500pg/mL
• Sensitivity: 4.688pg/mL

Mouse HSF2 ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Mouse HSF2 in samples. No significant cross-reactivity or interference between Mouse HSF2 and analogues was observed.
• Standard Curve Range: 7.813 - 500pg/mL
• Sensitivity: 4.688pg/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 4.9
• Intra-Assay: CV% < 5.02
• Spike Recovery: 86-105%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: HSF2

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Mouse HSF2 ELISA Kit (Colorimetric)

• heat shock factor 2
• heat shock factor protein 2
• heat shock transcription factor 2MGC75048
• HSF 2
• HSF2
• HSTF 2
• HSTF2
• MGC117376
• MGC156196

Background

Heat shock factor 1 (HSF1) is a heat shock transcription factor that activates the transcription of genes encoding products required for protein folding, processing, targeting, degradation, and function. Up-regulation of expression of heat shock proteins in response to stress occurs at the level of transcription through a heat shock element and a HSF transcription factor. Amino acid sequences for most HSFs are highly conserved. A DNA binding domain is at the N-terminus, hydrophobic repeats (essential to the formation of active trimers) are adjacent to this binding domain, and another short hydrophobic repeat (necessary for suppression of trimerization) occurs toward the C-terminus. HSF2 exists as two isoforms, the alpha form being more transcriptionally active than the smaller beta form. Various experiments have suggested that HSF2 may play roles in differentiation and development.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for HSF2 ELISA Kit (NBP2-76592). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: HSF2