Recombinant Human HSCB His Protein

SDS-Page: Recombinant Human HSCB Protein [NBP1-98925] - 15% SDS-PAGE (3ug)

• Reactivity: Hu
• Applications: PAGE
• Concentration: 1 mg/ml

Recombinant Human HSCB His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 30-235 of Human HSCB
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMAASQA GSNYPRCWNC GGPWGPGRED RFFCPQCRAL QAPDPTRDYF SLMDCNRSFR VDTAKLQHRY QQLQRLVHPD FFSQRSQTEK DFSEKHSTLV NDAYKTLLAP LSRGLYLLKL HGIEIPERTD YEMDRQFLIE IMEINEKLAE AESEAAMKEI ESIVKAKQKE FTDNVSSAFE QDDFEEAKEI LTKMRYFSNI EEKIKLKKIP L

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: HSCB
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 26.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH8.0), 10% glycerol, 0.15 M NaCl
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human HSCB His Protein

• DnaJ homolog subfamily C member 20
• DNAJC20DnaJ (Hsp40) homolog, subfamily C, member 20
• Hsc20
• HSC20dJ366L4.2
• HscB iron-sulfur cluster co-chaperone homolog (E. coli)
• iron-sulfur cluster co-chaperone protein HscB, mitochondrial
• JAC1
• J-type co-chaperone HSC20
• MGC2637
• MGC74462

Background

HSCB belongs to the hscB family and contains 1 J domain. This protein is expressed in lung, brain, stomach, spleen, ovary, testis, liver, muscle and heart and localized to mitochondria and cytoplasm. It may act as a co-chaperone in iron-sulfur cluster assembly in mitochondria, Interacts with ISCU and HSPA9. Recombinant human HSCB protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: HSCB