Recombinant Human HR6B/UBE2B Protein

Product Discontinued

Recombinant Human HR6B/UBE2B Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-152 of Human HR6B/UBE2B
  
  

  Source: E.coli

  Amino Acid Sequence: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMSTP ARRRLMRDFK RLQEDPPVGV SGAPSENNIM QWNAVIFGPE GTPFEDGTFK LVIEFSEEYP NKPPTVRFLS KMFHPNVYAD GSICLDILQN RWSPTYDVSS ILTSIQSLLD EPNPNSPANS QAAQLYQENK REYEKRVSAI VEQSWNDS

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: UBE2B
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 21.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH8.0), 10% glycerol, 0.1 M NaCl
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human HR6B/UBE2B Protein

• E2 protein
• E2-17k
• E2-17kDa
• HHR6B
• HR6B
• RAD6 homolog B
• RAD6BEC 6.3.2.19
• UBC2
• UBE2B
• Ubiquitin carrier protein B
• ubiquitin-conjugating enzyme E2 B
• Ubiquitin-conjugating enzyme E2-17 kDa
• ubiquitin-conjugating enzyme E2B (RAD6 homolog)
• Ubiquitin-protein ligase B

Background

UBE2B, also known as ubiquitin-conjugating enzyme E2 B, belongs to E2 ubiquitin-conjugating enzyme family. This protein catalyzes the covalent attachment of ubiquitin to other proteins, and associates to the E3 ligase RAD18 to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. It is required for postreplication repair of UV-damaged DNA. It may be involved in neurite outgrowth. Recombinant human UBE2B protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: UBE2B