HOXD10 Antibody

Immunohistochemistry: HOXD10 Antibody [NBP1-28476] - (3.8ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Flow Cytometry: HOXD10 Antibody [NBP1-28476] - Flow cytometric analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

• Reactivity: Hu, Mu, Rt, Bv, Eq
• Applications: ELISA, Flow, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

HOXD10 Antibody Summary

• Immunogen: Peptide with sequence PNRSCRIEQPVTQQ corresponding to internal region according to NP_002139.2.
• Epitope: internal region
• Predicted Species: Mouse (100%), Rat (100%), Equine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: HOXD10
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10ug/ml
  • Immunohistochemistry 3 - 5 ug/ml
  • Immunohistochemistry-Paraffin 3 - 5 ug/ml
  • Peptide ELISA Detection limit 1:32000
• Application Notes: WB: Approx. 37 kDa band observed in human kidney lysates (calculated MW of 38.4 kDa band according to NP_002139.2). Additional bands of unknown identity were also consistently observed at 90+26 kDa. These bands were successfully blocked by incubation with the immunizing peptide. IHC-P: Human kidney shows nuclear staining in some epithelial cells of PCT.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for HOXD10 Antibody

• homeo box 4D
• homeo box D10
• homeobox D10
• Homeobox protein Hox-4D
• Homeobox protein Hox-4E
• homeobox protein Hox-D10
• HOX4
• Hox-4.4
• HOX4D
• HOX4DHOX4
• HOX4E
• HOXD10

Background

This gene is a member of the Abd-B homeobox family and encodes a protein with a homeobox DNA-binding domain. It is included in a cluster of homeobox D genes located on chromosome 2. The encoded nuclear protein functions as a sequence-specific transcription factor that is expressed in the developing limb buds and is involved in differentiation and limb development. Mutations in this gene have been associated with Wilm's tumor and congenital vertical talus (also known as rocker-bottom foot deformity or congenital convex pes valgus) and/or a foot deformity resembling that seen in Charcot-Marie-Tooth disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HOXD10 Antibody (NBP1-28476)(2)

Publications using NBP1-28476

• Thamotharan S, Chu A, Kempf K et al. Differential microRNA expression in human placentas of term intra-uterine growth restriction that regulates target genes mediating angiogenesis and amino acid transport. PLoS ONE 2017-05-02 [PMID: 28463968]
• Myers C, Charboneau A, Cheung I et al. Sustained expression of homeobox D10 inhibits angiogenesis. Am J Pathol 2002-12-01 [PMID: 12466126]

Bioinformatics

• Gene Symbol: HOXD10
• Entrez:
  • 3236(Human)
  • 15430(Mouse)
  • 303991(Rat)
• Uniprot:
  • NP_002139.2(Human)