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HOXC10 Antibody

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Western Blot: HOXC10 Antibody [NBP1-71933] - Whole cell lysate from HeLa (15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), Jurkat (J; 50 mcg) and 293T (T; 50 mcg) cells. Affinity purified rabbit anti-HOXC10 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. HOXC10 was also immunoprecipitated by rabbit anti-HOXC10 antibody NBP1-71932, which recognizes an upstream epitope.
Independent Antibodies: Western Blot: HOXC10 Antibody [NBP1-71933] - Whole cell lysate from HeLa (15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), Jurkat (J; 50 mcg) and 293T (T; 50 mcg) cells. Affinity ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
 

Independent Antibodies

  

Biological Strategies

      

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HOXC10 Antibody Summary

Immunogen
The immunogen for this product maps to a region between residue 100 and 150 of human Homeobox C10 using the numbering given in entry NP_059105.2 (GeneID 3226).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HOXC10
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunoprecipitation 2-10 mcg/mg
  • Western Blot 1:2000-1:10000

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for HOXC10 Antibody

  • homeo box C10
  • homeobox C10
  • Homeobox protein Hox-3I
  • homeobox protein Hox-C10
  • homeoprotein C10
  • HOX3I
  • MGC5259

Background

This gene belongs to the homeobox family of genes. The homeobox genes encode a highly conserved family of transcription factors that play an important role in morphogenesis in all multicellular organisms. Mammals possess four similar homeobox gene clusters, HOXA, HOXB, HOXC and HOXD, which are located on different chromosomes and consist of 9 to 11 genes arranged in tandem. This gene is one of several homeobox HOXC genes located in a cluster on chromosome 12. The protein level is controlled during cell differentiation and proliferation, which may indicate this protein has a role in origin activation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Sandwich ELISA: HOXC13 Antibody (10D4) [H00003229-M01] - Detection limit for recombinant GST tagged HOXC13 is approximately 0.1ng/ml as a capture antibody.
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Immunocytochemistry/Immunofluorescence: HOXA9 Antibody [NBP2-32356] - Staining of human cell line HEK 293 shows localization to nucleus. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: HOXA9 Antibody [NBP2-32356] - Staining of human kidney shows strong nuclear positivity in cells in glomeruli.
Immunohistochemistry-Paraffin: HOXA9 Antibody [NBP2-32356] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.
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Western Blot: HOXA10 Antibody [NBP3-04877] - Analysis of extracts of Mouse kidney, using HOxA10 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit
Western Blot: HOXA10 Antibody [NBP3-04877] - Analysis of extracts of various cell lines, using HOxA10 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit
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Immunohistochemistry: HOXD10 Antibody (1D11) [NBP2-45744] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: HOXD10 Antibody (1D11) [NBP2-45744] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
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Western Blot: HOXC9 Antibody [NBP2-87596] - Host: Rabbit. Target Name: HOXC9. Sample Tissue: Human Jurkat Whole Cell. Antibody Dilution: 1ug/ml
Western Blot: HOXC9 Antibody [NBP2-87596] - WB Suggested Anti-HOXC9 Antibody Titration: 2.5ug/ml. Positive Control: Transfected 293T
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Flow Cytometry: Lamin B2 Antibody (2E2F4) [NBP2-61818] - Analysis of Hela cells using LMNB2 mouse mAb (green) and negative control (red).
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Western Blot: HOXB6 Antibody (8E3) [H00003216-M01-100ug] - Detection against Immunogen (32.01 KDa)
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ELISA: HOXC6 Antibody (2A4) [H00003223-M04] - Detection limit for recombinant GST tagged HOXC6 is 0.1 ng/ml as a capture antibody.
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Flow Cytometry: HOXC11 Antibody (3E10) [NBP2-00499] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-HOXC11 antibody, and then analyzed by flow cytometry.
Immunohistochemistry-Paraffin: HOXC11 Antibody (3E10) [NBP2-00499] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-HOXC11 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: HOXC11 Antibody (3E10) [NBP2-00499] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-HOXC11 mouse monoclonal antibody.
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FGF-8
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1 μg/lane of Recombinant Human/Mouse FGF-8b was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 23 kDa.
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Immunocytochemistry/Immunofluorescence: KMT2D Antibody [NBP1-89123] - Staining of human cell line A-431 shows localization to nucleus, plasma membrane & cytosol. Antibody staining is shown in green.
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Bioinformatics

Gene Symbol HOXC10
Uniprot