HOXA3 Overexpression Lysate

Images

 
Western Blot: HOXA3 Overexpression Lysate (Adult Normal) [NBL1-11667] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for HOXA3.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

HOXA3 Overexpression Lysate Summary

Description

HOXA3 Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC202355

Accession#: NM_030661

Protein Tag: C-MYC/DDK
Gene
HOXA3

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
46.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for HOXA3 Overexpression Lysate

  • homeo box A3
  • homeobox A3
  • Homeobox protein Hox-1E
  • homeobox protein Hox-A3
  • HOX1
  • Hox-1.5-like protein
  • HOX1Ehomeo box 1E
  • MGC10155

Background

In vertebrates, the genes encoding the class of transcription factors called homeobox genes are found in clusters named A, B, C, and D on four separate chromosomes. Expression of these proteins is spatially and temporally regulated during embryonic development. This gene is part of the A cluster on chromosome 7 and encodes a DNA-binding transcription factor which may regulate gene expression, morphogenesis, and differentiation. Three transcript variants encoding two different isoforms have been found for this gene. Transcript Variant: This variant (1) encodes the longer isoform (a). Variants 1 and 2 both encode isoform a.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-83054
Species: Hu
Applications: WB
H00003213-Q01
Species: Hu
Applications: ELISA, AP, PA, PAGE, WB
NB110-59723
Species: Hu, Mu, Po
Applications: ICC/IF, IHC, IHC-P, WB
H00003232-M09
Species: Hu
Applications: ELISA, ICC/IF, S-ELISA, WB
NBP2-20486
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, IP, WB
AF6318
Species: Hu
Applications: ICC
NBP2-46001
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
1845-SH/CF
Species: Hu
Applications: BA
H00003231-M01
Species: Hu
Applications: ELISA, IP, S-ELISA, WB
NBP2-24561
Species: Bv, Fe, Hu, Mu, Pm, Rt
Applications: IHC, IHC-P, WB
NBP2-45744
Species: Hu, Mu, Rt
Applications: IHC, WB
NBP3-13937
Species: Hu
Applications: ELISA, Flow, ICC/IF, IHC-P, IP, PA, WB
NBP3-12330
Species: Hu, Rt
Applications: ELISA, IHC, IHC-P, IP, WB
H00003217-M03
Species: Hu, Rb
Applications: ELISA, Func, IHC, IHC-P, WB
NBP2-56195
Species: Hu
Applications: ICC/IF, WB
NBP1-52085
Species: Hu, Mu
Applications: PEP-ELISA, WB
NBP2-87596
Species: Hu
Applications: IHC, IHC-P, WB
423-F8
Species: Hu, Mu
Applications: BA
NBP2-42520
Species: Hu, Po
Applications: IHC-P, PEP-ELISA, WB
NBL1-11667
Species: Hu
Applications: WB

Publications for HOXA3 Lysate (NBL1-11667) (0)

There are no publications for HOXA3 Lysate (NBL1-11667).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for HOXA3 Lysate (NBL1-11667) (0)

There are no reviews for HOXA3 Lysate (NBL1-11667). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for HOXA3 Lysate (NBL1-11667) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional HOXA3 Products

Bioinformatics Tool for HOXA3 Lysate (NBL1-11667)

Discover related pathways, diseases and genes to HOXA3 Lysate (NBL1-11667). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for HOXA3 Lysate (NBL1-11667)

Discover more about diseases related to HOXA3 Lysate (NBL1-11667).
 

Pathways for HOXA3 Lysate (NBL1-11667)

View related products by pathway.

PTMs for HOXA3 Lysate (NBL1-11667)

Learn more about PTMs related to HOXA3 Lysate (NBL1-11667).

Blogs on HOXA3

There are no specific blogs for HOXA3, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our HOXA3 Overexpression Lysate and receive a gift card or discount.

Bioinformatics

Gene Symbol HOXA3