hnRNP M Antibody

Immunocytochemistry/Immunofluorescence: hnRNP M Antibody [NBP2-97142] - Immunofluorescence staining of hnRNP M in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human hnRNP M polyclonal antibody (dilution ratio 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor$488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to nucleus.

Immunohistochemistry-Paraffin: hnRNP M Antibody [NBP2-97142] - Immunochemical staining of human hnRNP M in human pancreas with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.

Immunohistochemistry-Paraffin: hnRNP M Antibody [NBP2-97142] - Immunochemical staining of human hnRNP M in human duodenum with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.

Immunoprecipitation: hnRNP M Antibody [NBP2-97142] - hnRNP M was immunoprecipitated using: Lane A: 0.5 mg 293T Whole Cell Lysate Lane B: 0.5 mg HeLa Whole Cell Lysate Lane C: 0.5 mg Jurkat Whole Cell Lysate 4 ul anti-hnRNP M rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-hnRNP M rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 75 kDa. Observed band size: 75 kDa

Product Discontinued

hnRNP M Antibody Summary

• Description: This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Immunogen: Produced in rabbits immunized with E. coli-derived Human hnRNP M fragment.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HNRNPM
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 um filtered solution in PBS
• Preservative: No Preservative
• Purity: Antigen Affinity-purified

Alternate Names for hnRNP M Antibody

• DKFZp547H118
• heterogeneous nuclear ribonucleoprotein M
• hnRNP M
• HNRNPM4
• HNRPM4heterogenous nuclear ribonucleoprotein M
• HNRPMheterogenous nuclear ribonucleoprotein M4
• HTGR1
• M4 protein
• N-acetylglucosamine receptor 1
• NAGR1hnRNA-binding protein M4

Background

Proteins that directly bind to nascent RNA polymerase II transcripts--the heterogenous nuclear ribonucleoproteins (hnRNPs)--play an important role in both transcript-specific packaging and alternative splicing of pre-mRNAs. A group of abundant hnRNPs, the M1-M4 proteins, appear as a cluster of four proteins. The M proteins are pre-mRNA binding proteins in vivo, and they bind avidly to poly (G) and poly (U) RNA homopolymers in vitro. M proteins are members of the ribonucleoprotein consensus sequence family of RNA-binding proteins with greatest similarity to a hypothetical RNA-binding protein from Saccharomyces cerevisiae. The M proteins also possess an unusual hexapeptide-repeat region rich in methionine and arginine residues (MR repeat motif) that resembles a repeat in the 64kDa subunit of cleavage stimulation factor, which is involved in end maturation of pre-mRNAs. Proteins immunologically related to M exist in divergent eukaryotes ranging from human to yeast.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HNRNPM