HMGB2 Antibody (3E5) - Azide and BSA Free

Western Blot: HMGB2 Antibody (3E5) [H00003148-M05] - Analysis of HMGB2 expression in Hela S3 NE (Cat # L013V3).

Immunocytochemistry/Immunofluorescence: HMGB2 Antibody (3E5) [H00003148-M05] - Analysis of monoclonal antibody to HMGB2 on HeLa cell. Antibody concentration 10 ug/ml

Immunohistochemistry-Paraffin: HMGB2 Antibody (3E5) [H00003148-M05] - Analysis of monoclonal antibody to HMGB2 on formalin-fixed paraffin-embedded human testis. Antibody concentration 3 ug/ml

ELISA: HMGB2 Antibody (3E5) [H00003148-M05] - Detection limit for recombinant GST tagged HMGB2 is approximately 0.03ng/ml as a capture antibody.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 3E5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

HMGB2 Antibody (3E5) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: HMGB2 (AAH00903.2, 1 a.a. ~ 195 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MGKGDPNKPRGKMSSYAFFVQTCREEHKKKHPDSSVNFAEFSKKCSERWKTMSAKEKSKFEDMAKSDKARYDREMKNYVPPKGDKKGKKKDPNAPKRPPSAFFLFCSEHRPKIKSEHPGLSIGDTAKKLGEMWSEQSAKDKQPYEQKAAKLKEKYEKDIAAYRAKGKSEAGKKGPGRPTGSKKKNEPEDEEEEEE
• Specificity: HMGB2 (3E5)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: HMGB2
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF, IHC-P and ELISA.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 25284587). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for HMGB2 Antibody (3E5) - Azide and BSA Free

• High mobility group protein 2
• high mobility group protein B2
• high-mobility group box 2
• HMG-2
• HMG2high-mobility group (nonhistone chromosomal) protein 2

Background

This gene encodes a member of the non-histone chromosomal high mobility group protein family. The proteins of this family are chromatin-associated and ubiquitously distributed in the nucleus of higher eukaryotic cells. In vitro studies have demonstrated that this protein is able to efficiently bend DNA and form DNA circles. These studies suggest a role in facilitating cooperative interactions between cis-acting proteins by promoting DNA flexibility. This protein was also reported to be involved in the final ligation step in DNA end-joining processes of DNA double-strand breaks repair and V(D)J recombination.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HMGB2 Antibody (H00003148-M05)(1)

Publication using H00003148-M05

• Redmond Am, Byrne C, Bane Ft et al. Genomic interaction between ER and HMGB2 identifies DDX18 as a novel driver of endocrine resistance in breast cancer cells. Oncogene. 2014-10-06 [PMID: 25284587] (IHC-P, Mouse)

Bioinformatics

• Gene Symbol: HMGB2
• Entrez:
  • 3148(Human)
• OMIM:
  • 163906(Human)