HLA DRB3 293T Cell Transient Overexpression Lysate

Product Discontinued

HLA DRB3 293T Cell Transient Overexpression Lysate Summary

• Description: Quality control test: Transient overexpression cell lysate was tested with Anti-HLA-DRB3 antibody by Western Blots. Plasmid: pCMV-HLA-DRB3 full-length
• Specificity: HLA DRB3 293T Cell Transient Overexpression Lysate(Denatured)
• Gene: HLA-DRB3

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)

Lysate Details for Array

• Type: 293T Cell Transient Overexpression
• Protein State: Denatured

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for HLA DRB3 293T Cell Transient Overexpression Lysate

• DR7
• HLA class II histocompatibility antigen, DR beta 3 chain
• HLA class II histocompatibility antigen, DRB1-7 beta chain
• HLA-DR3B
• HLA-DR52
• HLA-DRB1
• human leucocyte antigen DRB3
• major histocompatibility complex, class II, DR beta 3
• MGC117330
• MHC class II antigen DR beta 3 chain
• MHC class II antigen DRB3
• MHC class II HLA-DR beta 3 chain

Background

HLA-DRB3 belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DRA) and a beta (DRB) chain, both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. DRB1 is expressed at a level five times higher than its paralogues DRB3, DRB4 and DRB5. The presence of DRB3 is linked with allelic variants of DRB1, otherwise it is omitted. There are 4 related pseudogenes: DRB2, DRB6, DRB7, DRB8 and DRB9. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: HLA-DRB3