HLA DRB1 Antibody (SPM423) [DyLight 680]

• Reactivity: Hu, Pm, Mu(-)
• Applications: WB, Flow, ICC/IF, IHC, ICC/IF
• Clone: SPM423
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: DyLight 680

HLA DRB1 Antibody (SPM423) [DyLight 680] Summary

• Description: This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
  
  For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
• Immunogen: Activated human peripheral blood mononuclear cells
• Localization: Cell Surface
• Specificity: This monoclonal antibody reacts with a 28kDa chain of HLA-DRB1 antigen, a member of MHC class II molecules. It does not cross react with HLA-DP and HLA-DQ. The L243 antibody recognizes a different epitope than the LN3 monoclonal antibody, and these antibodies do not cross-block binding to each others respective epitopes. HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36kDa alpha (heavy) chain and a 28kDa beta (light) chain. It is expressed on B-cells, activated T-cells, monocytes/macrophages, dendritic cells and other non-professional APCs. In conjunction with the CD3/TCR complex and CD4 molecules, HLA-DR is critical for efficient peptide presentation to CD4+ T cells. It is an excellent histiocytic marker in paraffin sections producing intense staining. True histiocytic neoplasms are similarly positive. HLA-DR antigens also occur on a variety of epithelial cells and their corresponding neoplastic counterparts. Loss of HLA-DR expression is related to tumor microenvironment and predicts adverse outcome in diffuse large B-cell lymphoma.
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: HLA-DRB1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
• Application Notes: Recommended applications are based on validated applications from the unconjugated base product NBP3-11552. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.

Reactivity Notes

Does not react with Mouse.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Notes

DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for HLA DRB1 Antibody (SPM423) [DyLight 680]

• Clone P2-beta-3
• DR1
• DR-1
• DR12
• DR-12
• DR13
• DR-13
• DR14
• DR-14
• DR16
• DR-16
• DR4
• DR-4
• DR5
• DR-5
• DR7
• DR-7
• DR8
• DR-8
• DR9
• DR-9
• DRB1
• DRw10
• DRw11
• DRw8
• DW2.2/DR2.2
• FLJ75017
• FLJ76359
• HLA class II antigen beta chain
• HLA class II histocompatibility antigen, DR-1 beta chain
• HLA-DR1B
• HLA-DRB
• HLA-DRB1
• HLA-DRB1*
• HLA-DRB2
• HLA-DR-beta 1
• human leucocyte antigen DRB1
• leucocyte antigen DR beta 1 chain
• leucocyte antigen DRB1
• lymphocyte antigen DRB1
• major histocompatibility complex, class II, DR beta 1
• MHC class II antigen DRB1*1
• MHC class II antigen DRB1*10
• MHC class II antigen DRB1*11
• MHC class II antigen DRB1*12
• MHC class II antigen DRB1*13
• MHC class II antigen DRB1*14
• MHC class II antigen DRB1*15
• MHC class II antigen DRB1*16
• MHC class II antigen DRB1*3
• MHC class II antigen DRB1*4
• MHC class II antigen DRB1*7
• MHC class II antigen DRB1*8
• MHC class II antigen DRB1*9
• MHC class II antigen HLA-DR13
• MHC class II HLA-DR beta 1 chain
• MHC class II HLA-DR-beta cell surface glycoprotein
• MHC class II HLA-DRw10-beta
• SS1

Background

HLA DRB1 can be used for HLA typing.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for HLA DRB1 Antibody (NBP3-11552FR). (Showing 1 - 1 of 1 FAQ).

1. We are interested in custom labeling our antibodies to be able to integrate new markers into already existing multicolor FACS panels. We would be glad to receive pricing and general order information for anti-HLA-DRB1 and anti-HLA-DRB5. To estimate expenses for future antibody labeling orders it would be helpful to know on what basis (amount, type, etc.) the price is going to be calculated. Right now the antibodies are still in mouse ascites. Would it be possible to integrate the elution of the antibodies from the ascites before labeling as part of the service agreement?
   • While we do not generally do this kind of custom purification unless it is for very large amounts of antibody, we do have a number of kits available that may be of use to you. Since an antibody that is still in ascites can not be labeled, it will need to be purified. A list of our kits available for this can be found at the following link: AbSelect. Once the antibodies are purified, they can be labeled with any number of different colors. A list of these kits for labeling can be found at the following link: Lightning-Link.

Bioinformatics

• Gene Symbol: HLA-DRB1