HLA DRA Antibody (19-26.1 (same as MB-26.1))

Immunohistochemistry-Paraffin: HLA DRA Antibody (19-26.1 (same as MB-26.1)) [NBP2-45044] - Human Tonsil stained with HLA-DRA Monoclonal Antibody (19-26.1).

Flow Cytometry: HLA DRA Antibody (19-26.1 (same as MB-26.1)) [NBP2-45044] - Flow Cytometric Analysis of Raji cells. HLA DRA Antibody (19-26.1 (same as MB-26.1)) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).

SDS-Page: HLA DRA Antibody (19-26.1 (same as MB-26.1)) [NBP2-45044]

• Reactivity: Hu
• Applications: Flow, IHC, IHC-P, PAGE, ICC/IF
• Clone: 19-26.1 (same as MB-26.1)
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

HLA DRA Antibody (19-26.1 (same as MB-26.1)) Summary

• Immunogen: Raji Cells
• Localization: Cell surface
• Specificity: This MAb reacts with the HLA-DR antigen, a member of MHC class II molecules. It does not cross react with HLA-DP and HLA-DQ. HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36kDa alpha (heavy) chain and a 28kDa beta (light) chain. It is expressed on B-cells, activated T-cells, monocytes/macrophages, dendritic cells and other non-professional APCs. In conjunction with the CD3/TCR complex and CD4 molecules, HLA-DR is critical for efficient peptide presentation to CD4+ T cells. It is an excellent histiocytic marker in paraffin sections producing intense cytoplasmic staining. True histiocytic neoplasms are similarly positive. HLA-DR antigens also occur on a variety of epithelial cells and their corresponding neoplastic counterparts.
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: HLA-DRA
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1 - 2 ug/ml
  • SDS-Page
  • Immunofluorescence 0.5 - 1.0 ug/ml
• Application Notes: Hu-chromosome location: 6p21.3
  Molecular weight of antigen: 36kDa (alpha chain) and 27kDa (beta chain)
  Immunohistochemistry-Paraffin 1 - 2 ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10 - 20 min followed by cooling at RT for 20 minutes. The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for HLA DRA Antibody (19-26.1 (same as MB-26.1))

• FLJ51114
• histocompatibility antigen HLA-DR alpha
• HLA class II histocompatibility antigen, DR alpha chain
• HLA-DRA1
• major histocompatibility complex, class II, DR alpha
• MHC cell surface glycoprotein
• MHC class II antigen DRA
• MLRW

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HLA-DRA