Histone H3 [Trimethyl Lys9] Antibody (6F12-H4)


Western Blot: Histone H3 [Trimethyl Lys9] Antibody (6F12-H4) [NBP1-30141] - Whole cell protein from human HeLa, A431, mouse Neuro2A and rat PC12 cells was separated on a 4-20% gel by SDS-PAGE, transferred to 0.2 um PVDF ...read more
Immunohistochemistry-Paraffin: Histone H3 [Trimethyl Lys9] Antibody (6F12-H4) [NBP1-30141] - Histone 3 was detected in immersion fixed paraffin-embedded sections of human colon cancer using anti-human mouse monoclonal ...read more
Immunocytochemistry/ Immunofluorescence: Histone H3 [Trimethyl Lys9] Antibody (6F12-H4) [NBP1-30141] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% ...read more
Western Blot: Histone H3 [Trimethyl Lys9] Antibody (6F12-H4) [NBP1-30141] - Analysis of Histone H3 (NBP1-30141) using HeLa nuclear lysate [NB800-PC9]. Observed molecular weight ~15 kDa.
Chromatin Immunoprecipitation: Histone H3 [Trimethyl Lys9] Antibody (6F12-H4) [NBP1-30141] - Used to perform ChIP on HeLa cells.
Western Blot: Histone H3 [Trimethyl Lys9] Antibody (6F12-H4) [NBP1-30141] - H3K9Me3 analysis of MCF7 and HCT116. Theorectical molecular weight is ~15 kDa. Image from verified customer review.
Western Blot: Histone H3 [Trimethyl Lys9] Antibody (6F12-H4) [NBP1-30141] - Representative Western blot showing M-track protein protein proximity signals obtained for Kic1. Hog1-protA-H3: background control, Nup2: ...read more
Western Blot: Histone H3 [Trimethyl Lys9] Antibody (6F12-H4) [NBP1-30141] - Analysis of Histone H3 K9-me3 in HeLa histone lysates. Observed molecular weight is ~15 kDa.

Product Details

Reactivity Hu, Mu, Rt, Po, Ce, Dr, In, Ma, YeSpecies Glossary
Applications WB, ChIP, ELISA, ICC/IF, IHC, IHC-P, IP
1 mg/ml

Order Details

Histone H3 [Trimethyl Lys9] Antibody (6F12-H4) Summary

This Histone H3 [Trimethyl Lys9] antibody (6F12-H4) was raised against a synthetic peptide made to an N-terminal region of Histone H3 (between amino acids 1-50). [UniProt# P84243]
Trimethyl Lys9
IgG1 Kappa
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Western Blot 1:2000
  • Chromatin Immunoprecipitation 1:10-1:500
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:200
  • Immunohistochemistry 1:1000
  • Immunohistochemistry-Paraffin 1:1000
  • Immunoprecipitation
Application Notes
In Western blot, a band is seen at ~15 kDa. Use in Immunohistochemistry reported in scientific literature (PMID: 23872316). Use in Immunoprecipitation reported in scientific literature (PMID: 28270554).
Theoretical MW
15 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
NBP1-30141 in the following applications:

Read Publications using
NBP1-30141 in the following applications:

  • 2 publications
  • 4 publications
  • IHC
    2 publications
  • 3 publications
  • IP
    1 publication
  • WB
    8 publications

Reactivity Notes

Predicted to react with most mammalian species. Invertebrate / Blattella germanica (German cockroach) reactivity reported in scientific literature (PMID: 23872316). Porcine reactivity reported in scientific literature (PMID: 25736622)

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Tris-Glycine and 0.15M NaCl
0.05% Sodium Azide
1 mg/ml
Protein G purified

Alternate Names for Histone H3 [Trimethyl Lys9] Antibody (6F12-H4)

  • H3 histone, family 3A
  • H3
  • H3.3A
  • H3.3B
  • H3F2
  • H3F3
  • H3F3A
  • H3F3B
  • H3FM
  • H3K9Me3
  • HIST2H3C
  • Histone H3
  • histone H3.3
  • MGC87782
  • MGC87783


Chromatin is an arrangement of DNA and proteins which form chromosomes. Chromatin is formed from nucleosomes, which are comprised of a set of four histone proteins (H2A, H2B, H3, H4) wrapped with DNA. Chromatin is very dynamic, where post-translational modifications can regulate DNA to be copied, transcribed, or repaired.

Histones are nuclear proteins responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Changes in chromatin structure play a large role in the regulation of transcription. The chromatin fibers are compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures.

Common histone modifications include methylation of lysine and arginine, acetylation of lysine, phosphorylation of threonine and serine, and sumoylation, biotinylation, and ubiquitylation of lysine. Posttranslational modifications such as acetylation of core histones regulates gene expression, thus altering protein function and regulation (1). Histone H3 is primarily acetylated at lysines 9, 14, 18, and 23 and have a theoretical molecular weight of 15 kDa. Acetylation at lysine 9 and 14 appears to control histone deposition, chromatin assembly and active transcription. Methylation of arginine residues within histone H3 has also been linked to transcription regulation. Histone H3 has been linked to various types of cancer as a biomarker through the aberrant expression of histone deacetylase (HDAC) enzymes and changes to chromatins (2-4).


1. Zhang, Y. X., Akumuo, R. C., Espana, R. A., Yan, C. X., Gao, W. J., & Li, Y. C. (2018). The histone demethylase KDM6B in the medial prefrontal cortex epigenetically regulates cocaine reward memory. Neuropharmacology, 141, 113-125. doi:10.1016/j.neuropharm.2018.08.030

2. Nandakumar, V., Hansen, N., Glenn, H. L., Han, J. H., Helland, S., Hernandez, K,...Meldrum, D. R. (2016). Vorinostat differentially alters 3D nuclear structure of cancer and non-cancerous esophageal cells. Sci Rep, 6, 30593. doi:10.1038/srep30593

3. Zhou, M., Li, Y., Lin, S., Chen, Y., Qian, Y., Zhao, Z., & Fan, H. (2019). H3K9me3, H3K36me3, and H4K20me3 Expression Correlates with Patient Outcome in Esophageal Squamous Cell Carcinoma as Epigenetic Markers. Dig Dis Sci, 64(8), 2147-2157. doi:10.1007/s10620-019-05529-2

4. Li, Y., Guo, D., Sun, R., Chen, P., Qian, Q., & Fan, H. (2019). Methylation Patterns of Lys9 and Lys27 on Histone H3 Correlate with Patient Outcome in Gastric Cancer. Dig Dis Sci, 64(2), 439-446. doi:10.1007/s10620-018-5341-8


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Histone H3 Antibody (NBP1-30141)(20)

We have publications tested in 6 confirmed species: Human, Mouse, Bacteria, Invertebrate, Porcine, Yeast.

We have publications tested in 6 applications: ChIP, ICC/IF, IHC, IHC-P, IP, WB.

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Showing Publications 1 - 10 of 20. Show All 20 Publications.
Publications using NBP1-30141 Applications Species
Saleh T, Alhesa A, Al-Balas M et al. Expression of therapy-induced senescence markers in breast cancer samples upon incomplete response to neoadjuvant chemotherapy Bioscience reports May 28 2021 [PMID: 33948615] (IHC, Human) IHC Human
Perea-GarcIa A, MirO P, JimEnez-Lorenzo R et al. Sequential recruitment of the mRNA decay machinery to the iron-regulated protein Cth2 in Saccharomyces cerevisiae Biochim Biophys Acta Gene Regul Mech Jun 19 2020 [PMID: 32565401] (WB, Yeast)

Saccharomyces cerevisia
WB Yeast
Qian Y, Li Y, Zheng C et al. High Methylation Levels of Histone H3 lysine 9 Associated with Activation of HIF-1 alpha Predict Patients’ Worse Prognosis in Human Hepatocellular Carcinomas Cancer Genetics May 1 2020 [PMID: 32534446] (IHC-P, Human) IHC-P Human
Hollenstein DM, GOmez-SAnchez R, Ciftci A Vac8 spatially confines autophagosome formation at the vacuole in S. cerevisiae J. Cell. Sci. Nov 14 2019 [PMID: 31649143] (WB, Yeast) WB Yeast
van Drogen F, Mishra R, Rudolf F et al. Mechanical stress impairs pheromone signaling via Pkc1-mediated regulation of the MAPK scaffold Ste5 J. Cell Biol. Jul 17 2019 [PMID: 31315942]
Janschitz M, Romanov N et al. Novel interconnections of HOG signaling revealed by combined use of two proteomic software packages. Cell Commun Signal Jun 17 2019 [PMID: 31208443] (WB, Human) WB Human
Zhou M, Li Y, Lin S et al. H3K9me3, H3K36me3, and H4K20me3 Expression Correlates with Patient Outcome in Esophageal Squamous Cell Carcinoma as Epigenetic Markers Dig. Dis. Sci. Feb 20 2019 [PMID: 30788686] (IHC-P, Human) IHC-P Human
Zhao C, Shi J, Zhou R et al. DZNep and UNC0642 enhance in vitro developmental competence of cloned pig embryos Reproduction Feb 1 2019 [PMID: 30730848] (ICC/IF, Porcine) ICC/IF Porcine
Stixova L, Komurkova D, Svobodova Kovarikova A, Bartova E. UVA irradiation strengthened an interaction between UBF1/2 proteins and H4K20 di-/tri-methylation. Chromosome Res. Jan 4 2019 [PMID: 30610403] (ICC/IF, WB, Mouse) ICC/IF, WB Mouse
Hollenstein D, Veis J, Romanov N et al. PP2ARts1 antagonizes Rck2-mediated hyperosmotic stress signaling in yeast bioRxiv May 31 2020 (WB, Bacteria) WB Bacteria
Show All 20 Publications.

Review for Histone H3 Antibody (NBP1-30141) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-30141:
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Images Ratings Applications Species Date Details
Western Blot Histone H3 NBP1-30141
reviewed by:
Verified Customer
WB Human 09/09/2014


ApplicationWestern Blot
Sample TestedH3K9(me)3 immunoblot in MCF7 and HCT116


Blocking DetailsNon-fat dry milk (5%) in PBS

Primary Anitbody

Dilution Ratio1:2000 in PBS at RT for 3 hours

Secondary Antibody

Secondary DescriptionAnti mouse IgG (HRP-conjugated)
Secondary Manufacturer Cat#ab6789 (Abcam)
Secondary Concentration1:5,000


Detection NotesBio-Rad Immun-Star HRP Substrate was used with X-ray film, Exposure time: 5sec

Product General Protocols

View specific protocols for Histone H3 Antibody (NBP1-30141): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for Histone H3 Antibody (NBP1-30141) (0)

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Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP1-30141AF350
Alexa Fluor 405 NBP1-30141AF405
Alexa Fluor 488 NBP1-30141AF488
Alexa Fluor 532 NBP1-30141AF532
Alexa Fluor 594 NBP1-30141AF594
Alexa Fluor 647 NBP1-30141AF647
Alexa Fluor 700 NBP1-30141AF700
Alexa Fluor 750 NBP1-30141AF750
Biotin NBP1-30141B
DyLight 350 NBP1-30141UV
DyLight 405 NBP1-30141V
DyLight 488 NBP1-30141G
DyLight 550 NBP1-30141R
DyLight 594 NBP1-30141DL594
DyLight 650 NBP1-30141C
DyLight 680 NBP1-30141FR
DyLight 755 NBP1-30141IR
FITC NBP1-30141F
HRP NBP1-30141H
Janelia Fluor 549 NBP1-30141JF549
Janelia Fluor 646 NBP1-30141JF646

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Research Areas for Histone H3 Antibody (NBP1-30141)

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Blogs on Histone H3.

Article Review: Glucose-induced transcriptional regulation in cancer
Epigenetic mechanisms have been implicated in many physiological and pathophysiological processes. Among these, histone modifications including methylation, phosphorylation, acetylation and ubiquitination, significantly modify gene expression. In c...  Read full blog post.

The role of DNMT3B in the co-incidence of methyltransferase and tumor suppressor expression in malignancies
Epigenetics is the process of heritable change in gene activity despite alteration of the hosts DNA sequence, essentially causing a change in a phenotype without a change in the genotype of a host. To change the gene sequence without interfering w...  Read full blog post.

The role of DNMT3A in development
Epigenetics is the study of heritable change in gene activity despite alteration of the hosts DNA sequence.  Change in gene activity done independently of the DNA sequence is achieved by way of histone and DNA methylation.  Gene silencing in DNA ...  Read full blog post.

EZH1 has more to offer than gene repression
EZH1 is part of the Polycomb-group family of proteins, which are responsible for remodeling chromatin in genes and modulating epigenetic silencing during development.  Specifically, EZHI is a component of PRC2, or polycomb repressive complex-2.  PR...  Read full blog post.

Understanding Transcription with RNA Polymerase II
RNA polymerase II is a large 12-subunit complex that synthesizes all mRNAs and several non-coding RNAs in eukaryotic cells. It is a DNA-dependent RNA polymerase enzyme that catalyzes transcription of DNA into RNA based on the four ribonucleoside triph...  Read full blog post.

Histone H3
Eukaryotic chromosomes are formed through the highly organized and structural wrapping of DNA genetic material around histone proteins into the classic "bead on a string" globular structure of nucleosomes. The histone family consists of five family me...  Read full blog post.

EZH2: Epigenetic Regulation Made Easy!
Enhancer of Zeste homolog 2 (EZH2) is the methyltransferase enzyme responsible for trimethylating lysine 27 on histone H3 to produce H3K27Me3. EZH2 is a polycomb group protein that is an essential epigenetic regulator that is often found deregulated i...  Read full blog post.

Understanding the Reasons for Histone H3 K4 Trimethylation (H3K4Me3)
Epigenetic mechanisms allow distinction between the active and inactive compartments of the genome, allowing proper cell lineage and embryogenesis. The trimethylation of Histone 3 at lysine 4 (H3K4Me3) is a common epigenetic histone modification that...  Read full blog post.

The 'epi-genie' is Out of the Bottle: Functional Histone 3 Variants in Human Disease
Discovery of histone variants using highly specific antibodies has led to the emerging notion that alterations in histone modifications and further changes in chromatin structure are induced by exchange of histone variants. Covalent histone modificati...  Read full blog post.

"Come Fly with Me" - New Drosophila Model Developed for Direct in Vivo Study of Histones
Forming the major protein component of chromatin, histones are essential to the structure and organization of chromosomes, forming the nucleosome around which DNA is packaged and wrapped.Antibody studies have revealed histones undergo various posttr...  Read full blog post.

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Recent Reviews


Verified Customer
Application: WB
Species: Human


Gene Symbol H3C14