Histone H2B, member S Antibody

Images

 
Western Blot: Histone H2B, member S Antibody [NB100-56347] - Doxycycline-inducible stable cell lines expressing TDP43 variants. Immunoblotting of whole-cell lysates and cytoplasmic and nuclear fractions for ...read more
Immunohistochemistry-Paraffin: Histone H2B, member S Antibody [NB100-56347] - Human heart stained with Histone H2B antibody at 10 ug/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 ...read more
Western Blot: Histone H2B, member S Antibody [NB100-56347] - Analysis of Histone H2B using NB100-56347 at 1:250 in nuclear and cytoplasmic fractions made from Drosophila head extracts using Novus' Nuclear Extraction ...read more
Western Blot: Histone H2B, member S Antibody [NB100-56347] - Top: Analysis of Histone H2B in human PBMC lysate with this antibody. Bottom: Western blot analysis of fractionated Cos-7 cells using this antibody ...read more
Western Blot: Histone H2B, member S Antibody [NB100-56347] - BAY-87-2243 inhibits nuclear HIF-1alpha protein expression. UT-SCC-5 tumors were treated either with BAY-87-2243 or carrier for 3, 5, or 7 days and were ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, Ch, Dr, Ma, ZeSpecies Glossary
Applications WB, IB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

Histone H2B, member S Antibody Summary

Immunogen
This antibody was generated by immunizing rabbits with a synthetic peptide corresponding to amino acid residues 111-125 AVSEGTKAVTKYTSS of human Histone H2B; GenBank no. CAI19747.1.
Specificity
A 15 kDa band is observed. This antibody is routinely quality controlled in human lysates.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
H2BFS
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunoblotting
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 10 ug/ml
  • Western Blot 2 ug/ml
Application Notes
Use in immunoblotting reported in scientific literature (PMID: 28235947).
Publications
Read Publications using
NB100-56347 in the following applications:

  • WB
    10 publications

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for Histone H2B, member S Antibody

  • H2B histone family S member10H2B/s
  • H2B histone family, member S
  • Histone H2B.s

Background

Histones are relatively small proteins that contain a high proportion of positively charged amino acids, e.g., arginine and lysine. This positive charge allows histones to bind tightly to the negatively charged DNA. There are five basic types of histone molecules called H1, H2A, H2B, H3, and H4. Histones are abundantly present in virtually all eukaryotic cells. Humsn histone H2B is a 125 amino acid protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Histone H2B, member S Antibody (NB100-56347)(14)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 1 application: WB.


Filter By Application
WB
(10)
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Filter By Species
Human
(5)
Mouse
(2)
Rat
(4)
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Showing Publications 1 - 10 of 14. Show All 14 Publications.
Publications using NB100-56347 Applications Species
Rieckhoff J, Meyer F, Classen S et al. Exploiting Chromosomal Instability of PTEN-Deficient Triple-Negative Breast Cancer Cell Lines for the Sensitization against PARP1 Inhibition in a Replication-Dependent Manner Cancers (Basel) Sep 29 2020 [PMID: 33003585] (WB, Human) WB Human
Paez-Colasante X, Figueroa-Romero C, Rumora AE et al. Cytoplasmic TDP43 Binds microRNAs: New Disease Targets in Amyotrophic Lateral Sclerosis Front Cell Neurosci May 12 2020 [PMID: 32477070] (WB, Human) WB Human
Malik AM, Miguez RA, Li X et al. Matrin 3-dependent neurotoxicity is modified by nucleic acid binding and nucleocytoplasmic localization Elife. 2018 Jul 16 [PMID: 30015619] (WB, Rat) WB Rat
Archbold HC, Jackson KL, Arora A et al. TDP43 nuclear export and neurodegeneration in models of amyotrophic lateral sclerosis and frontotemporal dementia. Sci Rep. Mar 15 2018 [PMID: 29545601] (Rat) Rat
Nechemia-Arbely Y, Fachinetti D, Miga KH et al. Human centromeric CENP-A chromatin is a homotypic, octameric nucleosome at all cell cycle points. J. Cell Biol. Feb 24 2017 [PMID: 28235947]
Myllynen L, Kwiatkowski M, Gleibner L et al. Quantitative proteomics unveiled: Regulation of DNA double strand break repair by EGFR involves PARP1. Radiother Oncol 2015 Sep 25 [PMID: 26422459] (WB, Human) WB Human
Chen SC, Huang SY, Lin WT et al. Aortic smooth muscle cell alterations in mice systemically exposed to arsenic Heart Vessels. 2015 Jul 02 [PMID: 26135927] (WB, Mouse) WB Mouse
Husted RF, Lu H, Sigmund RD, Stokes JB. Oxygen regulation of the epithelial Na channel in the collecting duct. Am J Physiol Renal Physiol. 2011 Feb [PMID: 21123494] (WB, Mouse)

Details:
IMGENEX product cited: Histone H2B (IMG-359). WB: Mouse collecting duct cells (mpkCCD-c14) and HEk-293 cells, data not shown.
WB Mouse
Qin Weiping, Pan Jiangping, Wu Yong et al. Protection against dexamethasone-induced muscle atrophy is related to modulation by testosterone of FOXO1 and PGC-1alpha. Biochem Biophys Res Commun. 2010 Dec 17 [PMID: 21094144] (Rat) Rat
Banerjee M, Datta M, Majumder P et al. Transcription regulation of caspase-1 by R393 of HIPPI and its molecular partner HIP-1. Nucleic Acids Res. 2010 Jan [PMID: 19934260] (WB)

Details:
WB: 1. Caspase-1 mAb (IMG-5028: aka IMG-804-4), Fig 3E (Hela cells) and Fig 5G (Neuro2A cells)
WB
Show All 14 Publications.

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Bioinformatics

Gene Symbol H2BFS
Entrez