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![Western Blot: Histone H2B, member S Antibody [NB100-56347] - Doxycycline-inducible stable cell lines expressing TDP43 variants. Immunoblotting of whole-cell lysates and cytoplasmic and nuclear fractions for alpha-tubulin (cytoplasmic marker; top panel), H2B (nuclear marker; middle panel), and eGFP (a marker for expression of the eGFP-His-tagged doxycycline-inducible plasmids) following subcellular fractionation of the control. Image collected and cropped by CiteAb from the following publication (//www.frontiersin.org/article/10.3389/fncel.2020.00117/full), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Histone-H2B--member-S-Antibody-Western-Blot-NB100-56347-img0011.jpg "Western Blot: Histone H2B, member S Antibody [NB100-56347] - Doxycycline-inducible stable cell lines expressing TDP43 variants. Immunoblotting of whole-cell lysates and cytoplasmic and nuclear fractions for alpha-tubulin (cytoplasmic marker; top panel), H2B (nuclear marker; middle panel), and eGFP (a marker for expression of the eGFP-His-tagged doxycycline-inducible plasmids) following subcellular fractionation of the control. Image collected and cropped by CiteAb from the following publication (//www.frontiersin.org/article/10.3389/fncel.2020.00117/full), licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: Histone H2B, member S Antibody [NB100-56347] - Human heart stained with Histone H2B antibody at 10 ug/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.](http://images.novusbio.com/fullsize/Histone-H2B--member-S-Antibody-Immunohistochemistry-Paraffin-NB100-56347-img0009.jpg)
![Western Blot: Histone H2B, member S Antibody [NB100-56347] - Analysis of Histone H2B using NB100-56347 at 1:250 in nuclear and cytoplasmic fractions made from Drosophila head extracts using Novus' Nuclear Extraction Kit. Data courtesy of Dr. Jerry Yin, University of Wisconsin-Madison.](http://images.novusbio.com/fullsize/Histone-H2B--member-S-Antibody-Western-Blot-NB100-56347-img0007.jpg)
![Western Blot: Histone H2B, member S Antibody [NB100-56347] - Top: Analysis of Histone H2B in human PBMC lysate with this antibody. Bottom: Western blot analysis of fractionated Cos-7 cells using this antibody anti-Histone H2B. Cos-7 cells were fractionated into nuclear cytosolic/membrane fractions. Approximately 15 ug of each fraction was separated on SDS PAGE. Lane 1) Nuclear fraction Lane 2) Cytosolic/membrane fraction.](http://images.novusbio.com/fullsize/Histone-H2B--member-S-Antibody-Western-Blot-NB100-56347-img0008.jpg)
![Western Blot: Histone H2B, member S Antibody [NB100-56347] - BAY-87-2243 inhibits nuclear HIF-1alpha protein expression. UT-SCC-5 tumors were treated either with BAY-87-2243 or carrier for 3, 5, or 7 days and were excised 24 h after end of treatment. Western blots of nuclear HIF-1alpha from different animals 3 days after BAY-87-2243 application. Quantification of western blots by densitometry relative to histone-H2B, means +/- SD. Image collected and cropped by CiteAb from the following publication (//ro-journal.biomedcentral.com/articles/10.1186/1748-717X-9-207), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Histone-H2B--member-S-Antibody-Western-Blot-NB100-56347-img0010.jpg)
![Western Blot: Histone H2B, member S [p Ser14] Antibody (C.15200186) [NBP2-62794] - HeLa cells were treated with colcemid to block the cell cycle in metaphase and histone extracts of these cells were analysed using the antibody against H2BS14p.](http://images.novusbio.com/fullsize/Histone-H2B-member-S-p-Ser14-Antibody-C-15200186-Western-Blot-NBP2-62794-img0004.jpg "Western Blot: Histone H2B, member S [p Ser14] Antibody (C.15200186) [NBP2-62794] - HeLa cells were treated with colcemid to block the cell cycle in metaphase and histone extracts of these cells were analysed using the antibody against H2BS14p.")
![Dot Blot: Histone H2B, member S [p Ser14] Antibody (C.15200186) [NBP2-62794] - To test the cross reactivity of the antibody against H2BS14p, an analysis was performed with peptides containing other histone phosphorylations and the unmodified H2BS14. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. This figure shows a high specificity of the antibody for the modification of interest.](http://images.novusbio.com/fullsize/Histone-H2B-member-S-p-Ser14-Antibody-C-15200186-Dot-Blot-NBP2-62794-img0003.jpg)
![ELISA: Histone H2B, member S [p Ser14] Antibody (C.15200186) [NBP2-62794] - To determine the titer of the antibody, analysis was performed using a serial dilution of the antibody against H2BS14p.The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:2,000.](http://images.novusbio.com/fullsize/Histone-H2B-member-S-p-Ser14-Antibody-C-15200186-ELISA-NBP2-62794-img0002.jpg)