Recombinant Human Histone H2AX His Protein

Western Blot: H2AX Protein [NBP1-42464] - The anti-H2AX antibody NB100-638 was used to detect this protein. 200ng of protein was loaded onto the gel and NB100-638 was used to detect the protein.

SDS-Page: H2AX Protein [NBP1-42464] - This H2AX protein was tested on a 4-20% SDS-PAGE gel with 5ug of protein loaded on the gel. The purity of the protein is >95%.

• Reactivity: Hu
• Applications: WB, PAGE
• Concentration: 0.5 mg/ml

Recombinant Human Histone H2AX His Protein Summary

• Description: Recombinant H2AX protein corresponding to amino acids 2 - 143.

  Source: E. coli

  (Accession #: P16104)

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: H2AX
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
  • Western Blot
• Application Notes: This product is useful as a positive control for both Western Blot and SDS-Page. This protein has been tested using NB100-638 for detection on a Western Blot.
• Theoretical MW: 17.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: 25 mM Tris pH 8.0, 250 mM NaCl, 8 M Urea, 1 mM DTT
• Preservative: N/A
• Concentration: 0.5 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human Histone H2AX His Protein

• H2A.X
• H2A/X
• H2AFX
• Histone H2AX

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. Human Histone H2AX with an N-terminal His tag expressed in E. coli and purified by conventional chromatography.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: H2AX