Histone H1T Antibody - Azide and BSA Free

Western Blot: Histone H1T Antibody [NBP3-15569] - Western blot analysis of extracts of various cell lines, using Histone H1T antibody (NBP3-15569) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Immunohistochemistry-Paraffin: Histone H1T Antibody [NBP3-15569] - Immunohistochemistry of paraffin-embedded rat lung using Histone H1T Rabbit pAb (NBP3-15569) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry-Paraffin: Histone H1T Antibody [NBP3-15569] - Immunohistochemistry of paraffin-embedded human esophageal using Histone H1T Rabbit pAb (NBP3-15569) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry-Paraffin: Histone H1T Antibody [NBP3-15569] - Immunohistochemistry of paraffin-embedded mouse kidney using Histone H1T Rabbit pAb (NBP3-15569) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Histone H1T Antibody - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit Histone H1T Antibody - Azide and BSA Free (NBP3-15569) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 150-207 of human Histone H1t (NP_005314.2). NKRAKKPRATTPKTVRSGRKAKGAKGKQQQKSPVKARASKSKLTQHHEVNVRKATSKK
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: H1-6
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500 - 1:2000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.01% Thimerosal
• Purity: Affinity purified

Alternate Names for Histone H1T Antibody - Azide and BSA Free

• dJ221C16.2
• H1 histone family, member T (testis-specific)
• H1FTMGC163222
• H1t
• histone 1, H1t
• histone cluster 1, H1t
• histone H1t
• Testicular H1 histone

Background

Histones are nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber as they play a critical role in transcription regulation, DNA repair, DNA replication, and chromosomal stability. Histone H1t is a 207 amino acid long, 22 kDA protein encoded by the gene HIST1H1T that lacks polyA tails and instead obtain palindromic termination elements. H1 histones are critical for the condensation of nucleosome chains into higher order structures. HIST1H1T is found on chromosome 5. HIST1H1T has been investigated in childhood leukemia and hemochromatosis. It is involved in granzyme-A pathway, histone modification, and PKA signaling and is known to interact with various genes such as YWHAQ, YWHAZ, ACTA2, POU5F1, and GSK3B.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

Bioinformatics

• Gene Symbol: H1-6